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http://hdl.handle.net/2445/178669
Title: | Epithelial–Mesenchymal Transition (EMT) Induced by TGF-β in Hepatocellular Carcinoma Cells Reprograms Lipid Metabolism |
Author: | Soukupova, Jitka Malfettone, Andrea Bertran Rodríguez, Esther Hernández Álvarez, María Isabel Peñuelas Haro, Irene Dituri, Francesco Giannelli, Gianluigi Zorzano Olarte, Antonio Fabregat Romero, Isabel |
Keywords: | Càncer de fetge Oxidació fisiològica Liver cancer Physiological oxidation |
Issue Date: | 24-May-2021 |
Publisher: | MDPI |
Abstract: | (1) Background: The transforming growth factor (TGF)-β plays a dual role in liver carcinogenesis. At early stages, it inhibits cell growth and induces apoptosis. However, TGF-β expression is high in advanced stages of hepatocellular carcinoma (HCC) and cells become resistant to TGF-β induced suppressor effects, responding to this cytokine undergoing epithelial-mesenchymal transition (EMT), which contributes to cell migration and invasion. Metabolic reprogramming has been established as a key hallmark of cancer. However, to consider metabolism as a therapeutic target in HCC, it is necessary to obtain a better understanding of how reprogramming occurs, which are the factors that regulate it, and how to identify the situation in a patient. Accordingly, in this work we aimed to analyze whether a process of full EMT induced by TGF-β in HCC cells induces metabolic reprogramming. (2) Methods: In vitro analysis in HCC cell lines, metabolomics and transcriptomics. (3) Results: Our findings indicate a differential metabolic switch in response to TGF-β when the HCC cells undergo a full EMT, which would favor lipolysis, increased transport and utilization of free fatty acids (FFA), decreased aerobic glycolysis and an increase in mitochondrial oxidative metabolism. (4) Conclusions: EMT induced by TGF-β in HCC cells reprograms lipid metabolism to facilitate the utilization of FFA and the entry of acetyl-CoA into the TCA cycle, to sustain the elevated requirements of energy linked to this process. |
Note: | Reproducció del document publicat a: https://doi.org/10.3390/ijms22115543 |
It is part of: | International Journal of Molecular Sciences, 2021, vol. 22 |
URI: | http://hdl.handle.net/2445/178669 |
Related resource: | https://doi.org/10.3390/ijms22115543 |
Appears in Collections: | Articles publicats en revistes (Bioquímica i Biomedicina Molecular) Articles publicats en revistes (Ciències Fisiològiques) Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) Articles publicats en revistes (Institut de Recerca Biomèdica (IRB Barcelona)) |
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