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http://hdl.handle.net/2445/178788
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DC Field | Value | Language |
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dc.contributor.author | Lawal, Opeyemi U. | - |
dc.contributor.author | Barata, Marta | - |
dc.contributor.author | Fraqueza, Maria J. | - |
dc.contributor.author | Worning, Peder | - |
dc.contributor.author | Bartels, Mette D. | - |
dc.contributor.author | Goncalves, Luisa | - |
dc.contributor.author | Paixao, Paulo | - |
dc.contributor.author | Goncalves, Elsa | - |
dc.contributor.author | Toscano, Cristina | - |
dc.contributor.author | Empel, Joanna | - |
dc.contributor.author | Urbas, Malgorzata | - |
dc.contributor.author | Domínguez Luzón, Ma. Ángeles (María Ángeles) | - |
dc.contributor.author | Westh, Henrik | - |
dc.contributor.author | Lencastre, Hermínia de | - |
dc.contributor.author | Miragaia, María | - |
dc.date.accessioned | 2021-07-05T12:10:25Z | - |
dc.date.available | 2021-07-05T12:10:25Z | - |
dc.date.issued | 2021-06-07 | - |
dc.identifier.issn | 1664-302X | - |
dc.identifier.uri | http://hdl.handle.net/2445/178788 | - |
dc.description.abstract | Biofilm formation has been shown to be critical to the success of uropathogens. Although Staphylococcus saprophyticus is a common cause of urinary tract infections, its biofilm production capacity, composition, genetic basis, and origin are poorly understood. We investigated biofilm formation in a large and diverse collection of S. saprophyticus (n = 422). Biofilm matrix composition was assessed in representative strains (n = 63) belonging to two main S. saprophyticus lineages (G and S) recovered from human infection, colonization, and food-related environment using biofilm detachment approach. To identify factors that could be associated with biofilm formation and structure variation, we used a pangenome-wide association study approach. Almost all the isolates (91%; n = 384/422) produced biofilm. Among the 63 representative strains, we identified eight biofilm matrix phenotypes, but the most common were composed of protein or protein-extracellular DNA (eDNA)-polysaccharides (38%, 24/63 each). Biofilms containing protein-eDNA-polysaccharides were linked to lineage G and environmental isolates, whereas protein-based biofilms were produced by lineage S and infection isolates (p < 0.05). Putative biofilm-associated genes, namely, aas, atl, ebpS, uafA, sasF, sasD, sdrH, splE, sdrE, sdrC, sraP, and ica genes, were found with different frequencies (3-100%), but there was no correlation between their presence and biofilm production or matrix types. Notably, icaC_1 was ubiquitous in the collection, while icaR was lineage G-associated, and only four strains carried a complete ica gene cluster (icaADBCR) except one that was without icaR. We provided evidence, using a comparative genomic approach, that the complete icaADBCR cluster was acquired multiple times by S. saprophyticus and originated from other coagulase-negative staphylococci. Overall, the composition of S. saprophyticus biofilms was distinct in environmental and clinical isolates, suggesting that modulation of biofilm structure could be a key step in the pathogenicity of these bacteria. Moreover, biofilm production in S. saprophyticus is ica-independent, and the complete icaADBCR was acquired from other staphylococci. | - |
dc.format.extent | 13 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Frontiers Media | - |
dc.relation.isformatof | Reproducció del document publicat a: https://doi.org/10.3389/fmicb.2021.663768 | - |
dc.relation.ispartof | Frontiers in Microbiology, 2021, vol. 12, num. 663768 | - |
dc.relation.uri | https://doi.org/10.3389/fmicb.2021.663768 | - |
dc.rights | cc-by (c) Lawal, Opeyemi U. et al., 2021 | - |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | - |
dc.source | Articles publicats en revistes (Patologia i Terapèutica Experimental) | - |
dc.subject.classification | Estafilococs | - |
dc.subject.classification | Infeccions del tracte urinari | - |
dc.subject.classification | Epidemiologia | - |
dc.subject.other | Staphylococcus | - |
dc.subject.other | Urinary tract infections | - |
dc.subject.other | Epidemiology | - |
dc.title | Staphylococcus saprophyticus from clinical and environmental origins have distinct biofilm composition | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.idgrec | 711936 | - |
dc.date.updated | 2021-07-05T12:10:26Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 34163443 | - |
Appears in Collections: | Articles publicats en revistes (Patologia i Terapèutica Experimental) Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) |
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File | Description | Size | Format | |
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711936.pdf | 1.21 MB | Adobe PDF | View/Open |
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