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Title: AMPAR/TARP stoichiometry differentially modulates channel properties
Author: Miguez Cabello, Federico
Sánchez Fernández, Núria
Yefimenko Nosova, Natalia
Gasull Casanova, Xavier
Gratacòs i Batlle, Esther
Soto del Cerro, David
Keywords: Electrofisiologia
Neural transmission
Issue Date: 26-May-2020
Publisher: eLife Sciences
Abstract: AMPARs control fast synaptic communication between neurons and their function relies on auxiliary subunits, which importantly modulate channel properties. Although it has been suggested that AMPARs can bind to TARPs with variable stoichiometry, little is known about the effect that this stoichiometry exerts on certain AMPAR properties. Here we have found that AMPARs show a clear stoichiometry-dependent modulation by the prototypical TARP γ2 although the receptor still needs to be fully saturated with γ2 to show some typical TARP-induced characteristics (i.e. an increase in channel conductance). We also uncovered important differences in the stoichiometric modulation between calcium-permeable and calcium-impermeable AMPARs. Moreover, in heteromeric AMPARs, γ2 positioning in the complex is important to exert certain TARP-dependent features. Finally, by comparing data from recombinant receptors with endogenous AMPAR currents from mouse cerebellar granule cells, we have determined a likely presence of two γ2 molecules at somatic receptors in this cell type.
Note: Reproducció del document publicat a:
It is part of: eLife, 2020, vol. 9, num. e53946
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ISSN: 2050-084X
Appears in Collections:Articles publicats en revistes (Biomedicina)

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