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Title: In Situ LSPR Sensing of Secreted Insulin in Organ-on-Chip
Author: Ortega, María Alejandra
Rodríguez Comas, Júlia
Ozlem, Yavas
Velasco Mallorquí, Ferran
Balaguer Trias, Jordina
Parra, Victor
Novials, Anna
Servitja, Joan M.
Quidant, Romain
Ramón Azcón, Javier
Keywords: Biosensors
Issue Date: 28-Apr-2021
Publisher: MDPI
Abstract: Organ-on-a-chip (OOC) devices offer new approaches for metabolic disease modeling and drug discovery by providing biologically relevant models of tissues and organs in vitro with a high degree of control over experimental variables for high-content screening applications. Yet, to fully exploit the potential of these platforms, there is a need to interface them with integrated non-labeled sensing modules, capable of monitoring, in situ, their biochemical response to external stimuli, such as stress or drugs. In order to meet this need, we aim here to develop an integrated technology based on coupling a localized surface plasmon resonance (LSPR) sensing module to an OOC device to monitor the insulin in situ secretion in pancreatic islets, a key physiological event that is usually perturbed in metabolic diseases such as type 2 diabetes (T2D). As a proof of concept, we developed a biomimetic islet-on-a-chip (IOC) device composed of mouse pancreatic islets hosted in a cellulose-based scaffold as a novel approach. The IOC was interfaced with a state-of-the-art on-chip LSPR sensing platform to monitor the in situ insulin secretion. The developed platform offers a powerful tool to enable the in situ response study of microtissues to external stimuli for applications such as a drug-screening platform for human models, bypassing animal testing.
Note: Reproducció del document publicat a:
It is part of: Biosensors 2021, vol. 11, num. 5, p.138
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Appears in Collections:Articles publicats en revistes (IDIBAPS: Institut d'investigacions Biomèdiques August Pi i Sunyer)
Articles publicats en revistes (Institut de Bioenginyeria de Catalunya (IBEC))

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