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Title: | TP53-Induced Glycolysis and Apoptosis Regulator (TIGAR) Is Upregulated in Lymphocytes Stimulated with Concanavalin A |
Author: | Simon Molas, Helga Vallvé-Martínez, Xavier Caldera-Quevedo, Irene Fontova, Pere Arnedo-Pac, Claudia Vidal Alabró, Anna Castaño Boldú, Esther Navarro i Sabaté, Àurea Lloberas Blanch, Núria Bartrons Bach, Ramon Manzano Cuesta, Anna |
Keywords: | Endocrinologia molecular Molecular endocrinology |
Issue Date: | 11-Jul-2021 |
Publisher: | MDPI |
Abstract: | The glycolytic modulator TP53-Inducible Glycolysis and Apoptosis Regulator (TIGAR) is overexpressed in several types of cancer and has a role in metabolic rewiring during tumor development. However, little is known about the role of this enzyme in proliferative tissues under physiological conditions. In the current work, we analysed the role of TIGAR in primary human lymphocytes stimulated with the mitotic agent Concanavalin A (ConA). We found that TIGAR expression was induced in stimulated lymphocytes through the PI3K/AKT pathway, since Akti-1/2 and LY294002 inhibitors prevented the upregulation of TIGAR in response to ConA. In addition, suppression of TIGAR expression by siRNA decreased the levels of the proliferative marker PCNA and increased cellular ROS levels. In this model, TIGAR was found to support the activity of glucose 6-phosphate dehydrogenase (G6PDH), the first enzyme of the pentose phosphate pathway (PPP), since the inhibition of TIGAR reduced G6PDH activity and increased autophagy. In conclusion, we demonstrate here that TIGAR is upregulated in stimulated human lymphocytes through the PI3K/AKT signaling pathway, which contributes to the redirection of the carbon flux to the PPP. |
Note: | Reproducció del document publicat a: https://doi.org/10.3390/ijms22147436 |
It is part of: | International Journal of Molecular Sciences, 2021, vol. 22, num. 14, p. 7436 |
URI: | http://hdl.handle.net/2445/179842 |
Related resource: | https://doi.org/10.3390/ijms22147436 |
ISSN: | 1661-6596 |
Appears in Collections: | Articles publicats en revistes (Infermeria Fonamental i Clínica) Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) |
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