Please use this identifier to cite or link to this item:
http://hdl.handle.net/2445/180597
Title: | Differences in activity and functional connectivity between bi-dimensional and three-dimensional neuronal cultures |
Author: | Barrantes Cepas, Mar |
Director/Tutor: | Soriano i Fradera, Jordi |
Keywords: | Cultiu cel·lular Xarxes neuronals (Neurobiologia) Treballs de fi de grau Cell culture Neural networks (Neurobiology) Bachelor's theses |
Issue Date: | Jul-2021 |
Abstract: | Three-dimensional (3D) neuronal cultures can provide a more desirable in vitro ap-proach to emulate brain-like conditions. To prove it, 2D and 3D neuronal cultures were compared. During different days in vitro (DIV) after formation, spontaneous neuronal activity was recorded through calcium fluorescence imaging for data extraction. We show that activity in 3D cultures is higher and more varied. These cultures are functionally organized in modules that are spatially localized. In comparison, 2D cultures show less variability in activity, higher connectivity between neurons and a poor organization in modules. We ascribe the differences to the capacity of 3D cul-tures to form connections of short distance, favouring small microcircuits that coexist, while the 2D cultures have long–range connections that shape a strongly coupled and rigid system |
Note: | Treballs Finals de Grau de Física, Facultat de Física, Universitat de Barcelona, Curs: 2021, Tutor: Jordi Soriano Fradera |
URI: | http://hdl.handle.net/2445/180597 |
Appears in Collections: | Treballs Finals de Grau (TFG) - Física |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
BARRANTES CEPAS MAR_4253552_assignsubmission_file_TFG-Barrantes-Cepas-Mar.pdf | 2.34 MB | Adobe PDF | View/Open |
This item is licensed under a Creative Commons License