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http://hdl.handle.net/2445/181016
Title: | Inhibition of CBP synergizes with the RNA-dependent mechanisms of Azacitidine by limiting protein synthesis |
Author: | Diesch, Jeannine Pannérer, Marguerite Marie Le Winkler, René Casquero, Raquel Muhar, Matthias Van Der Garde, Mark Maher, Michael Martínez Herráez, Carolina Bech Serra, Joan J. Fellner, Michaela Rathert, Philipp Brooks, Nigel Zamora, Lurdes Gentilella, Antonio Torre, Carolina de la Zuber, Johannes Götze, Katharina S. Buschbeck, Marcus |
Keywords: | Hematopoesi Terapèutica Hematopoiesis Therapeutics |
Issue Date: | 18-Oct-2021 |
Publisher: | Springer Science and Business Media LLC |
Abstract: | The nucleotide analogue azacitidine (AZA) is currently the best treatment option for patients with high-risk myelodysplastic syndromes (MDS). However, only half of treated patients respond and of these almost all eventually relapse. New treatment options are urgently needed to improve the clinical management of these patients. Here, we perform a loss-of-function shRNA screen and identify the histone acetyl transferase and transcriptional co-activator, CREB binding protein (CBP), as a major regulator of AZA sensitivity. Compounds inhibiting the activity of CBP and the closely related p300 synergistically reduce viability of MDS-derived AML cell lines when combined with AZA. Importantly, this effect is specific for the RNA-dependent functions of AZA and not observed with the related compound decitabine that is only incorporated into DNA. The identification of immediate target genes leads us to the unexpected finding that the effect of CBP/p300 inhibition is mediated by globally down regulating protein synthesis. © 2021. The Author(s). |
Note: | Reproducció del document publicat a: https://doi.org/10.1038/s41467-021-26258-z |
It is part of: | Nature Communications, 2021, vol. 12, num. 1 |
URI: | http://hdl.handle.net/2445/181016 |
Related resource: | https://doi.org/10.1038/s41467-021-26258-z |
ISSN: | 2041-1723 |
Appears in Collections: | Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) |
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s41467-021-26258-z.pdf | 1.26 MB | Adobe PDF | View/Open |
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