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http://hdl.handle.net/2445/181306
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DC Field | Value | Language |
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dc.contributor.author | Sánchez, Aránzazu | - |
dc.contributor.author | Álvarez Barrientos, Alberto | - |
dc.contributor.author | Benito, Manuel | - |
dc.contributor.author | Fabregat Romero, Isabel | - |
dc.date.accessioned | 2021-11-17T14:04:58Z | - |
dc.date.available | 2021-11-17T14:04:58Z | - |
dc.date.issued | 1996-03-29 | - |
dc.identifier.issn | 0021-9258 | - |
dc.identifier.uri | http://hdl.handle.net/2445/181306 | - |
dc.description.abstract | Transforming growth factor-beta (TGF-beta), a growth regulator of fetal hepatocytes in primary culture, also regulates death of these cells. Dose-response analysis showed that the TGF-beta concentration needed to induce hepatocyte death (2.5 ng/ml) was 5 times that needed to inhibit growth in these cells (0.5 ng/ml). In response to TGF-beta, hepatocytes induced DNA fragmentation and the appearance of nuclei with a DNA content lower than 2C (diploid content), typical of a programmed cell death model. TGF-beta-induced apoptosis in fetal hepatocytes was preceded by an induction of reactive oxygen species production and a decrease in the glutathione intracellular content, indicating that this factor induces oxidative stress in fetal hepatocytes. Studies performed to analyze levels of c-fos mRNA, a gene whose expression is modulated by redox state, demonstrated that only high, apoptotic concentrations of TGF-beta (2.5 ng/ml) produced an increase in the mRNA levels of this gene, the level of induction being similar to that found when cells were incubated in the presence of tert-butyl hydroperoxide. Gel mobility shift assays showed that the c-fos-induced expression was coincident with an increase in AP-1 activity. Finally, cell death induced by TGF-beta in fetal hepatocytes was partially blocked by radical scavengers, which decreased the percentage of apoptotic cells, whereas these agents did not modify the growth-inhibitory effect elicited by TGF-beta in these cells. In summary, the results presented in this paper provide evidence for the involvement of an oxidative process in the apoptosis elicited by TGF-beta in fetal hepatocytes. | - |
dc.format.extent | 7 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | American Society for Biochemistry and Molecular Biology | - |
dc.relation.isformatof | Reproducció del document publicat a: https://doi.org/10.1074/jbc.271.13.7416 | - |
dc.relation.ispartof | Journal of Biological Chemistry, 1996, vol. 271, num. 13, p. 7416-7422 | - |
dc.relation.uri | https://doi.org/10.1074/jbc.271.13.7416 | - |
dc.rights | (c) American Society for Biochemistry and Molecular Biology, 1996 | - |
dc.source | Articles publicats en revistes (Ciències Fisiològiques) | - |
dc.subject.classification | Apoptosi | - |
dc.subject.classification | Efectes secundaris dels medicaments | - |
dc.subject.classification | Fetge | - |
dc.subject.classification | Metabolisme | - |
dc.subject.other | Apoptosis | - |
dc.subject.other | Drug side effects | - |
dc.subject.other | Liver | - |
dc.subject.other | Metabolism | - |
dc.title | Apoptosis induced by transforming growth factor-β in fetal hepatocyte primary cultures | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.idgrec | 579693 | - |
dc.date.updated | 2021-11-17T14:04:58Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 8631767 | - |
Appears in Collections: | Articles publicats en revistes (Ciències Fisiològiques) |
Files in This Item:
File | Description | Size | Format | |
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579693.pdf | 359.26 kB | Adobe PDF | View/Open |
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