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Title: | Multi-laboratory experiment PME11 for the standardization of phosphoproteome analysis |
Author: | Colomé, Núria Abián, Joaquín Aloria, Kerman Arizmendi, Jesús M. Barceló Batllori, Sílvia Braga Lagache, Sophie Burlet Schiltz, Odile Carrascal, Montse Casal, J. Ignacio Chicano Gálvez, Eduard Chiva, Cristina Clemente, Luis Felipe Elortza, Felix Estanyol i Ullate, Josep Maria Fernandez Irigoyen, Joaquín Fernández Puente, Patricia Fidalgo, María José Froment, Carine Fuentes, Manuel Fuentes Almagro, Carlos Gay, Marina Hainard, Alexandre Heller, Manfred Hernández, María Luisa Ibarrola, Nieves Iloro, Ibon Kieselbach, Thomas Lario, Antonio Locard Paulet, Marie Marina Ramírez, Anabel Martín, Luna Morato López, Esperanza Muñoz, Javier Navajas, Rosana Odena, M. Antonia Odriozola, Leticia Oliveira, Eliandre Paradela, Alberto Pasquarello Mosimann, Carla Rios, Vivian de los Ruiz Romero, Cristina Sabidó Aguadé, Eduard Sánchez del Pino, Manuel Sancho, Jaime Santamaría, Enrique Schaeffer Reiss, Christine Schneider, Justine Torre, Carolina de la Valero, M. Luz Vilaseca, Marta Wu, Shuai Wu, Linfeng Ximénez Embún, Pilar Canals, Francesc Corrales, Fernando |
Keywords: | Proteòmica Normalització Proteomics Standardization |
Issue Date: | 1-Nov-2021 |
Publisher: | Elsevier BV |
Abstract: | Global analysis of protein phosphorylation by mass spectrometry proteomic techniques has emerged in the last decades as a powerful tool in biological and biomedical research. However, there are several factors that make the global study of the phosphoproteome more challenging than measuring non-modified proteins. The low stoichiometry of the phosphorylated species and the need to retrieve residue specific information require particular attention on sample preparation, data acquisition and processing to ensure reproducibility, qualitative and quantitative robustness and ample phosphoproteome coverage in phosphoproteomic workflows. Aiming to investigate the effect of different variables in the performance of proteome wide phosphoprotein analysis protocols, ProteoRed-ISCIII and EuPA launched the Proteomics Multicentric Experiment 11 (PME11). A reference sample consisting of a yeast protein extract spiked in with different amounts of a phosphomix standard (Sigma/Merck) was distributed to 31 laboratories around the globe. Thirty-six datasets from 23 laboratories were analyzed. Our results indicate the suitability of the PME11 reference sample to benchmark and optimize phosphoproteomics strategies, weighing the influence of different factors, as well as to rank intra and inter laboratory performance. |
Note: | Reproducció del document publicat a: https://doi.org/10.1016/j.jprot.2021.104409 |
It is part of: | Journal of Proteomics, 2021, vol. 251 |
URI: | http://hdl.handle.net/2445/181798 |
Related resource: | https://doi.org/10.1016/j.jprot.2021.104409 |
ISSN: | 1874-3919 |
Appears in Collections: | Articles publicats en revistes (Centres Científics i Tecnològics de la Universitat de Barcelona (CCiTUB)) Articles publicats en revistes (IDIBAPS: Institut d'investigacions Biomèdiques August Pi i Sunyer) Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) Articles publicats en revistes (Institut de Recerca Biomèdica (IRB Barcelona)) |
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