Please use this identifier to cite or link to this item:
http://hdl.handle.net/2445/182834
Title: | Conditional expression of PfAP2-G for controlled massive sexual conversion in Plasmodium falciparum |
Author: | Llorà Batlle, Oriol Michel Todó, Lucas Witmer, Kathrin Toda, Haruka Fernández Becerra, Carmen Baum, Jake Cortés, Alfred |
Keywords: | Malària Microbiologia mèdica Malaria Medical microbiology |
Issue Date: | 2020 |
Publisher: | American Association for the Advancement of Science |
Abstract: | --- - i: - Plasmodium falciparum content: - "Malaria transmission requires that some asexual parasites convert into sexual forms termed gametocytes. The initial stages of sexual development, including sexually committed schizonts and sexual rings, remain poorly haracterized, mainly because they are morphologically identical to their sexual counterparts and only a small subset of parasites undergo sexual development. Here, we describe a system for controlled sexual conversion in the human malaria parasite " - ", based on conditional expression of the PfAP2-G transcription factor. Using this system, ~90 percent of the parasites converted into sexual forms upon induction, enabling the characterization of committed and early sexual stages without further purification. We characterized sexually committed schizonts and sexual rings at the transcriptomic and phenotypic levels, which revealed down-regulation of genes involved in solute transport upon sexual commitment, among other findings. The new inducible lines will facilitate the study of early sexual stages at additional levels, including multiomic characterization and drug susceptibility assays." |
Note: | Reproducció del document publicat a: http://dx.doi.org/10.1126/sciadv.aaz5057 |
It is part of: | Science advances, 2020, vol 6, num 24 |
URI: | http://hdl.handle.net/2445/182834 |
Related resource: | http://dx.doi.org/ 10.1126/sciadv.aaz5057 |
ISSN: | 2375-2548 |
Appears in Collections: | Articles publicats en revistes (ISGlobal) |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Llora_Batlle_O_Sci_Adv_2020.pdf | 1.07 MB | Adobe PDF | View/Open |
This item is licensed under a Creative Commons License