Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/183399
Title: Astrocytic BDNF and TrkB regulate severity and neuronal activity in mouse models of temporal lobe epilepsy
Author: Fernández, Sara (Fernández García)
Sancho Balsells, Anna
Longueville, Sophie
Hervé, Denis
Gruart i Massó, Agnès
Delgado García, José M.
Alberch i Vié, Jordi, 1959-
Giralt Torroella, Albert
Keywords: Models animals en la investigació
Astròcits
Epilèpsia
Animal models in research
Astrocytes
Epilepsy
Issue Date: 1-Jun-2020
Publisher: Nature Publishing Group
Abstract: Astrocytes have emerged as crucial regulators of neuronal network activity, synapse formation, and underlying behavioral and cognitive processes. Despite some pathways have been identified, the communication between astrocytes and neurons remains to be completely elucidated. Unraveling this communication is crucial to design potential treatments for neurological disorders like temporal lobe epilepsy (TLE). The BDNF and TrkB molecules have emerged as very promising therapeutic targets. However, their modulation can be accompanied by several off-target effects such as excitotoxicity in case of uncontrolled upregulation or dementia, amnesia, and other memory disorders in case of downregulation. Here, we show that BDNF and TrkB from astrocytes modulate neuronal dysfunction in TLE models. First, conditional overexpression of BDNF from astrocytes worsened the phenotype in the lithium-pilocarpine mouse model. Our evidences pointed out to the astrocytic pro-BDNF isoform as a major player of this altered phenotype. Conversely, specific genetic deletion of BDNF in astrocytes prevented the increase in the number of firing neurons and the global firing rate in an in vitro model of TLE. Regarding to the TrkB, we generated mice with a genetic deletion of TrkB specifically in hippocampal neurons or astrocytes. Interestingly, both lines displayed neuroprotection in the lithium-pilocarpine model but only the mice with genetic deletion of TrkB in astrocytes showed significantly preserved spatial learning skills. These data identify the astrocytic BDNF and TrkB molecules as promising therapeutic targets for the treatment of TLE.
Note: Reproducció del document publicat a: https://doi.org/10.1038/s41419-020-2615-9
It is part of: Cell Death and Disease, 2020, vol. 11, num. 6, p. 411
URI: http://hdl.handle.net/2445/183399
Related resource: https://doi.org/10.1038/s41419-020-2615-9
ISSN: 2041-4889
Appears in Collections:Articles publicats en revistes (IDIBAPS: Institut d'investigacions Biomèdiques August Pi i Sunyer)
Articles publicats en revistes (Biomedicina)

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