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http://hdl.handle.net/2445/183467| Title: | Immunostaining Protocol: P-Stat3 (Xenograft and Mice) |
| Author: | Calon, Alexandre Espinet, Elisa Palomo Ponce, Sergio Tauriello, Daniele V. F. Iglesias, Mar Cespedes, María Virtudes Sevillano, Marta Nadal, Cristina Jung, Peter Zhang, Xiang H. F. Byrom, Daniel Riera i Escalé, Antoni Rossell, David Mangues, Ramon Massagué i Solé, Joan, 1953- Sancho, Elena Batlle, Eduard |
| Keywords: | Càncer colorectal Metàstasi Expressió gènica Colorectal cancer Metastasis Gene expression |
| Issue Date: | 5-Mar-2014 |
| Abstract: | We sought to understand the mechanisms behind the potent effect of stromal TGF-beta program on the capacity of colorectal cancer (CRC) cells to initiate metastasis. We discovered that mice subcutaneous tumors and metastases generated in the context of a TGF-beta activated microenvironment displayed prominent accumulation of p-STAT3 in CRC cells compared with those derived from control cells. STAT3 signaling depended on GP130 as shown by strong reduction of epithelial p STAT3 levels upon GP130 shRNA-mediated knockdown in CRC cells. |
| Note: | Reproducció del document publicat a: https://doi.org/10.21769/BioProtoc.1120 |
| It is part of: | Bio-protocol, 2014, vol. 4, num. 9, p. e1120 |
| URI: | http://hdl.handle.net/2445/183467 |
| Related resource: | https://doi.org/10.21769/BioProtoc.1120 |
| ISSN: | 2331-8325 |
| Appears in Collections: | Articles publicats en revistes (Institut de Recerca Biomèdica (IRB Barcelona)) |
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| File | Description | Size | Format | |
|---|---|---|---|---|
| Bio-protocol1120.pdf | 440.08 kB | Adobe PDF | View/Open |
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