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Title: | A descriptive analysis of urinary ESBL-Producing-Escherichia coli in Cerdanya Hospital |
Author: | Gaviria, Lorena Patrícia Montsant, Lourdes Azuaje, Carlos González-Díaz, Aida Horcajada Gallego, Juan Pablo Limón, Enrique Viñas, Miquel Espinal, Paula Fusté i Domínguez, Ester |
Keywords: | Escheríchia coli Infeccions del tracte urinari Resistència als medicaments Escherichia coli Urinary tract infections Drug resistance |
Issue Date: | 18-Feb-2022 |
Publisher: | MDPI |
Abstract: | Urinary tract infections caused by extended-spectrum β-lactamase Escherichia coli (ESBLEC) are increasing worldwide and are a current concern because treatment options are often limited. This study investigated antimicrobial susceptibility, antimicrobial resistance genes (ARGs), and the biological diversity of urinary ESBL-EC isolates at Cerdanya Hospital, a European cross-border hospital that combines French and Spanish healthcare models. Bacterial identification and susceptibility were determined using the Microscan WalkAway® system and ESBL production was examined by the double-disk synergy method. Isolates were sequenced using the Ion S5¿ next-generation sequencing system, with the whole-genome sequences then assembled using SPADEs software and analyzed using PubMLST, ResFinder, FimTyper, PlasmidFinder, and VirulenceFinder. A phylogenetic analysis was performed by constructing an assembly-based core-SNV alignment, followed by a phylogenetic tree constructed using Parsnp from the Harvest suite. All isolates studied were multidrug-resistant and could be classified into 19 different sequence types characterized by a high genetic diversity. The most prevalent ESBL-enzymes were CTX-M-14 and CTX-M-15. High-risk international clones (ST131, ST10, and ST405) were also identified. The results demonstrated the absence of a single predominant clone of ESBL-MDR-EC at Cerdanya Hospital. |
Note: | Reproducció del document publicat a: https://doi.org/10.3390/microorganisms10030488 |
It is part of: | Microorganisms, 2022, vol. 10, p. 488 |
URI: | http://hdl.handle.net/2445/183506 |
Related resource: | https://doi.org/10.3390/microorganisms10030488 |
ISSN: | 2076-2607 |
Appears in Collections: | Articles publicats en revistes (Infermeria de Salut Pública, Salut mental i Maternoinfantil) Articles publicats en revistes (Patologia i Terapèutica Experimental) Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) |
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