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http://hdl.handle.net/2445/183522| Title: | Immunostaining Protocol: P-Smad2 (Xenograft and Mice) |
| Author: | Calon, Alexandre Espinet, Elisa Palomo Ponce, Sergio Tauriello, Daniele V. F. Iglesias, Mar Céspedes, María Sevillano, Marta Nadal, Cristina Jung, Peter Zhang, Xiang Byrom, Daniel Riera i Escalé, Antoni Rossell, David Mangues, Ramon Massagué i Solé, Joan, 1953- Sancho, Elena Batlle, Eduard |
| Keywords: | Càncer colorectal Metàstasi Expressió gènica Colorectal cancer Metastasis Gene expression |
| Issue Date: | 5-May-2014 |
| Abstract: | Metastasis depends on a gene program expressed by the tumor microenvironment upon TGF-beta stimulation. CRC (Colorectal cancer) cell lines did not induce robust stromal TGF- beta responses when injected into nude mice as shown by lack of p- SMAD2 accumulation in tumor-associated stromal cells. To enforce high TGF-beta signaling in xenografts, we engineered CRC cell lines to secrete active TGF-beta. Subcutaneous tumors obtained from HT29-M6TGF-β, KM12L4aTGF-β cells and SW48TGF-β cells contained abundant p-SMAD2+ stromal cells. |
| Note: | Reproducció del document publicat a: https://doi.org/10.21769/bioprotoc.1119 |
| It is part of: | Bio-protocol, 2014, vol. 4, num. 9, p. e1119 |
| URI: | http://hdl.handle.net/2445/183522 |
| Related resource: | https://doi.org/10.21769/bioprotoc.1119 |
| ISSN: | 2331-8325 |
| Appears in Collections: | Articles publicats en revistes (Institut de Recerca Biomèdica (IRB Barcelona)) |
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| File | Description | Size | Format | |
|---|---|---|---|---|
| Bio-protocol1119.pdf | 522.96 kB | Adobe PDF | View/Open |
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