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Title: | Transplantation of Human Induced Pluripotent Stem Cell-Derived Retinal Pigment Epithelium in a Swine Model of Geographic Atrophy |
Author: | Duarri, Anna Rodríguez Bocanegra, Eduardo Martínez Navarrete, Gema Biarnés, Marc García, Miriam Lee Ferraro, Lucía Kuebler, B. Aran Corbella, Begoña Izquierdo, Elisabeth Aguilera Xiol, Elisabet Casaroli Marano, Ricardo Pedro Trias, Esteve Fernández, Eduardo Raya Chamorro, Ángel Veiga, Anna Monés, Jordi |
Keywords: | Trasplantament d'òrgans Retina Models animals en la investigació Transplantation of organs Retina Animal models in research |
Issue Date: | 28-Sep-2021 |
Publisher: | MDPI |
Abstract: | Background: The aim of this study was to test the feasibility and safety of subretinal transplantation of human induced pluripotent stem cell (hiPSC)-derived retinal pigment epithelium (RPE) cells into the healthy margins and within areas of degenerative retina in a swine model of geographic atrophy (GA). Methods: Well-delimited selective outer retinal damage was induced by subretinal injection of NaIO3 into one eye in minipigs (n = 10). Thirty days later, a suspension of hiPSC-derived RPE cells expressing green fluorescent protein was injected into the subretinal space, into the healthy margins, and within areas of degenerative retina. In vivo follow-up was performed by multimodal imaging. Post-mortem retinas were analyzed by immunohistochemistry and histology. Results: In vitro differentiated hiPSC-RPE cells showed a typical epithelial morphology, expressed RPE-related genes, and had phagocytic ability. Engrafted hiPSC-RPE cells were detected in 60% of the eyes, forming mature epithelium in healthy retina extending towards the border of the atrophy. Histological analysis revealed RPE interaction with host photoreceptors in the healthy retina. Engrafted cells in the atrophic zone were found in a patchy distribution but failed to form an epithelial-like layer. Conclusions: These results might support the use of hiPSC-RPE cells to treat atrophic GA by providing a housekeeping function to aid the overwhelmed remnant RPE, which might improve its survival and therefore slow down the progression of GA. Keywords: age-related macular degeneration (AMD); geographic atrophy; pig; animal model; stem cells; iPSC; RPE; retina; regenerative medicine; advanced cell therapy |
Note: | Reproducció del document publicat a: https://doi.org/10.3390/ijms221910497 |
It is part of: | International Journal of Molecular Sciences, 2021, vol. 22, num. 19, p. 10497 |
URI: | http://hdl.handle.net/2445/183749 |
Related resource: | https://doi.org/10.3390/ijms221910497 |
ISSN: | 1661-6596 |
Appears in Collections: | Articles publicats en revistes (Cirurgia i Especialitats Medicoquirúrgiques) Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) |
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