Please use this identifier to cite or link to this item:
http://hdl.handle.net/2445/183786
Title: | Endothelial junctional membrane protrusions serve as hotspots for neutrophil transmigration |
Author: | Arts, Janine J.G. Mahlandt, Elike K. Grönloh, Max L.B. Schimmel, Lilian Noordstra, Ivar Gordon, Emma van Steen, Abraham C.I. Tol, Simon Walzog, Barbara van Rijssel, Jos Nolte, Martijn A. Postma, Marten Khuon, Satya Heddleston, John M. Wait, Eric Chew, Teng Leong Winter, Mark Montañez, Eloi Goedhart, Joachim Van Buul, Jaap D. |
Keywords: | Endoteli Leucòcits Inflamació Immunologia Endothelium Leucocytes Inflammation Immunology |
Issue Date: | 25-Aug-2021 |
Publisher: | eLife Sciences |
Abstract: | Upon inflammation, leukocytes rapidly transmigrate across the endothelium to enter the inflamed tissue. Evidence accumulates that leukocytes use preferred exit sites, alhough it is not yet clear how these hotspots in the endothelium are defined and how they are recognized by the leukocyte. Using lattice light sheet microscopy, we discovered that leukocytes prefer endothelial membrane protrusions at cell junctions for transmigration. Phenotypically, these junctional membrane protrusions are present in an asymmetric manner, meaning that one endothelial cell shows the protrusion and the adjacent one does not. Consequently, leukocytes cross the junction by migrating underneath the protruding endothelial cell. These protrusions depend on Rac1 activity and by using a photo-activatable Rac1 probe, we could artificially generate local exit-sites for leukocytes. Overall, we have discovered a new mechanism that uses local induced junctional membrane protrusions to facilitate/steer the leukocyte escape/exit from inflamed vessel walls. |
Note: | Reproducció del document publicat a: https://doi.org/10.7554/eLife.66074 |
It is part of: | eLife, 2021 |
URI: | http://hdl.handle.net/2445/183786 |
Related resource: | https://doi.org/10.7554/eLife.66074 |
ISSN: | 2050-084X |
Appears in Collections: | Articles publicats en revistes (Ciències Fisiològiques) |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
720998.pdf | 7.15 MB | Adobe PDF | View/Open |
This item is licensed under a Creative Commons License