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Title: High within-host diversity found from direct genotyping on post-mortem tuberculosis specimens in a high-burden setting
Author: Rodríguez Grande, Cristina
Hurtado, Juan Carlos
Rodríguez Maus, Sandra
Casas, Isaac
Castillo, Paola
Navarro, Mireia
Rakislova, Natalia
García Basteiro, Alberto L.erto
Carrilho, Carla
Fernandes, Fabiola
Lovane, Lucilia
Jordao, Dercio
Ismail, Mamudo Rafik
Lorenzoni, Cesaltina
Cossa, Anelsio
Mandomando, Inácio
Bassat Orellana, Quique
Menéndez, Clara
Ordi i Majà, Jaume
Muñoz, Patricia
Pérez Lago, Laura
García de Viedma, Darío
Martínez Yoldi, Miguel Julián
Keywords: Mycobacterium tuberculosis
Àfrica oriental
Genètica bacteriana
Mycobacterium tuberculosis
East Africa
Bacterial genetics
Issue Date: 11-Jun-2021
Publisher: European Society of Clinical Microbiology and Infectious Diseases
Abstract: Objectives: To characterize the clonal complexity in Mycobacterium tuberculosis (MTB) infections considering factors that help maximize the detection of coexisting strains/variants. Methods: Genotypic analysis by Mycobacterial Interspersed Repetitive-Unit-Variable-Number Tandem-Repeats (MIRU-VNTR) was performed directly on 70 biopsy specimens from two or more different tissues involving 28 tuberculosis cases diagnosed post-mortem in Mozambique, a country with a high tuberculosis burden. Results: Genotypic data from isolates collected from two or more tissues were obtained for 23 of the 28 cases (82.1%), allowing the analysis of within-patient diversity. MIRU-VNTR analysis revealed clonal diversity in ten cases (35.7%). Five cases showed allelic differences in three or more loci, suggesting mixed infection with two different strains. In half of the cases showing within-host diversity, one of the specimens associated with clonal heterogeneity was brain tissue. Conclusions: Direct MTB genotyping from post-mortem tissue samples revealed a frequent within-host Mycobacterium tuberculosis diversity, including mixed and polyclonal infections. Most of this diversity would have been overlooked if only standard analysis of respiratory specimens had been performed.
Note: Reproduccío del document publicat a:
It is part of: Clinical Microbiology and Infection, 2021, vol. 27, num. 10, p. 1518
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ISSN: 1198-743X
Appears in Collections:Articles publicats en revistes (Fonaments Clínics)

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