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Title: | Involvement of Bax protein in the prevention of glucocorticoid-induced thymocytes apoptosis by melatonin |
Author: | Hoijman Kleinman, Esteban Rocha Viegas, Luciana Keller Sarmiento, María Inés Rosenstein, Ruth E. Pecci, Adali |
Keywords: | Apoptosi Melatonina Glucocorticoides Proteïnes Apoptosis Melatonin Glucocorticoids Proteins |
Issue Date: | Jan-2004 |
Publisher: | Association for the Study of Internal Secretions |
Abstract: | The antiapoptotic effect of melatonin has been described in several systems. In this study, the antagonistic effect of the methoxyindole on dexamethasone-induced apoptosis in mouse thymocytes was examined. Melatonin decreased both DNA fragmentation, and the number of annexin V-positive cells incubated in the presence of dexamethasone. Analysis of the expression of the members of the Bcl-2 family indicated that the synthetic glucocorticoid increased Bax protein levels without affecting the levels of Bcl-2, Bcl-XL, Bcl-XS, or Bak. This effect correlated with an increase in thymocytes bax mRNA levels. Dexamethasone also increased the release of cytochrome C from mitochondria. All of these effects were reduced in the presence of melatonin, which was ineffective per se on these parameters. In addition, the involvement of cAMP on glucocorticoid/melatonin antagonism was examined. Both melatonin and dexamethasone decreased the levels of this nucleotide in mouse thymocytes, indicating that the antagonistic action between both hormones involves a cAMP-independent pathway. In summary, the present results suggest that the antiapoptotic effect of melatonin on glucocorticoid-treated thymocytes would be a consequence of an inhibition of the mitochondrial pathway, presumably through the regulation of Bax protein levels. |
Note: | Reproducció del document publicat a: https://doi.org/10.1210/en.2003-0764 |
It is part of: | Endocrinology, 2004, vol. 145, num. 1, p. 418-425 |
URI: | http://hdl.handle.net/2445/186609 |
Related resource: | https://doi.org/10.1210/en.2003-0764 |
ISSN: | 0013-7227 |
Appears in Collections: | Articles publicats en revistes (Patologia i Terapèutica Experimental) |
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