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Title: Comprehensive assessment of myocardial remodeling in ischemic heart disease by synchrotron propagation based X-ray phase contrast imaging.
Author: Planinc, Ivo
Garcia Canadilla, Patricia
Dejea, Hector
Ilic, Ivana
Guasch i Casany, Eduard
Zamora, Monica
Crispi Brillas, Fàtima
Stampanoni, Marco
Milicic, Davor
Bijnens, Bart
Bonnin, Anne
Cikes, Maja
Keywords: Diagnòstic per la imatge
Raigs X
Diagnostic imaging
Issue Date: 7-Jan-2021
Publisher: Nature Publishing Group
Abstract: Cardiovascular research is in an ongoing quest for a superior imaging method to integrate grossanatomical information with microanatomy, combined with quantifable parameters of cardiac structure. In recent years, synchrotron radiation-based X-ray Phase Contrast Imaging (X-PCI) has been extensively used to characterize soft tissue in detail. The objective was to use X-PCI to comprehensively quantify ischemic remodeling of diferent myocardial structures, from cell to organ level, in a rat model of myocardial infarction. Myocardial infarction-induced remodeling was recreated in a well-established rodent model. Ex vivo rodent hearts were imaged by propagation based X-PCI using two confgurations resulting in 5.8 µm and 0.65 µm efective pixel size images. The acquired datasets were used for a comprehensive assessment of macrostructural changes including the whole heart and vascular tree morphology, and quantifcation of left ventricular myocardial thickness, mass, volume, and organization. On the meso-scale, tissue characteristics were explored and compared with histopathological methods, while microstructural changes were quantifed by segmentation of cardiomyocytes and calculation of cross-sectional areas. Propagation based X-PCI provides detailed visualization and quantifcation of morphological changes on whole organ, tissue, vascular as well as individual cellular level of the ex vivo heart, with a single, non-destructive 3D imaging modality.
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It is part of: Scientific Reports, 2021, vol. 11, p. 14020
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ISSN: 2045-2322
Appears in Collections:Articles publicats en revistes (IDIBAPS: Institut d'investigacions Biomèdiques August Pi i Sunyer)
Articles publicats en revistes (Medicina)

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