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Title: | A C˄S-cyclometallated gold(III) complex as novel antibacterial candidate against drug-resistant bacteria |
Author: | Ratia, Carlos Cepas, Virginio Soengas, Raquel Navarro, Yolanda Velasco de Andrés, María Iglesias, Maria José Lozano, Francisco López Ortiz, Fernando Soto, Sara M. |
Keywords: | Resistència als medicaments Medicaments antibacterians Crisoteràpia Drug resistance Antibacterial agents Chrysotherapy |
Issue Date: | 3-Mar-2022 |
Publisher: | Frontiers Media |
Abstract: | The worldwide emergence and spread of infections caused by multidrug-resistant bacteria endangers the efficacy of current antibiotics in the clinical setting. The lack of new antibiotics in the pipeline points to the need of developing new strategies. Recently, gold-based drugs are being repurposed for antibacterial applications. Among them, gold(III) complexes have received increasing attention as metal-based anticancer agents. However, reports on their antibacterial activity are scarce due to stability issues. The present work demonstrates the antibacterial activity of the gold(III) complex 2 stabilized as C∧S-cycloaurated containing a diphenylphosphinothioic amide moiety, showing minimum inhibitory concentration (MIC) values that ranged from 4 to 8 and from 16 to 32 mg/L among Gram-positive and Gram-negative multidrug-resistant (MDR) pathogens, respectively. Complex 2 has a biofilm inhibitory activity of only two to four times than its MIC. We also describe for the first time a potent antibacterial synergistic effect of a gold(III) complex combined with colistin, showing a bactericidal effect in less than 2 h; confirming the role of the outer membrane as a permeability barrier. Complex 2 shows a low rate of internalization in Staphylococcus aureus and Acinetobacter baumannii; it does not interact with replication enzymes or efflux pumps, causes ultrastructural damages in both membrane and cytoplasmic levels, and permeabilizes the bacterial membrane. Unlike control antibiotics, complex 2 did not generate resistant mutants in 30-day sequential cultures. We detected lower cytotoxicity in a non-tumoral THLE-2 cell line (IC50 = 25.5 μM) and no acute toxicity signs in vivo after an i.v. 1-mg/kg dose. The characterization presented here reassures the potential of complex 2 as a new chemical class of antimicrobial agents. |
Note: | Reproducció del document publicat a: https://doi.org/10.3389/fmicb.2022.815622 |
It is part of: | Frontiers in Microbiology, 2022, vol. 13 |
URI: | http://hdl.handle.net/2445/191021 |
Related resource: | https://doi.org/10.3389/fmicb.2022.815622 |
ISSN: | 1664-302X |
Appears in Collections: | Articles publicats en revistes (IDIBAPS: Institut d'investigacions Biomèdiques August Pi i Sunyer) Articles publicats en revistes (Biomedicina) |
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