Please use this identifier to cite or link to this item:
http://hdl.handle.net/2445/192657
Title: | Host-dependent editing of SARS-CoV-2 in COVID-19 patients |
Author: | Gregori, Josep Cortese, Maria Francesca Piñana, Maria Campos, Carolina Garcia-Cehic, Damir Andrés, Cristina Abril Ferrando, Josep Francesc, 1970- Codina, Maria Gema Rando, Ariadna Esperalba, Juliana Sulleiro, Elena Joseph, Joan Saubí, Narcís Colomer-Castell, Sergi Martin, Mari Carmen Castillo, Carla Esteban, Juan Ignacio Pumarola Suñé, Tomàs Rodríguez-Frías, Francisco Antón, Andrés Quer, Josep |
Keywords: | SARS-CoV-2 Mutació (Biologia) Genoma humà COVID-19 SARS-CoV-2 Mutation (Biology) Human genome COVID-19 |
Issue Date: | 1-Dec-2021 |
Publisher: | Taylor and Francis |
Abstract: | A common trait among RNA viruses is their high capability to acquire genetic variability due to viral and host mechanisms. Next-generation sequencing (NGS) analysis enables the deep study of the viral quasispecies in samples from infected individuals. In this study, the viral quasispecies complexity and single nucleotide polymorphisms of the SARS-CoV-2 spike gene of coronavirus disease 2019 (COVID-19) patients with mild or severe disease were investigated using next-generation sequencing (Illumina platform). SARS-CoV-2 spike variability was higher in patients with long-lasting infection. Most substitutions found were present at frequencies lower than 1%, and had an A → G or T → C pattern, consistent with variants caused by adenosine deaminase acting on RNA-1 (ADAR1). ADAR1 affected a small fraction of replicating genomes, but produced multiple, mainly non-synonymous mutations. ADAR1 editing during replication rather than the RNA-dependent RNA polymerase (nsp12) was the predominant mechanism generating SARS-CoV-2 genetic variability. However, the mutations produced are not fixed in the infected human population, suggesting that ADAR1 may have an antiviral role, whereas nsp12-induced mutations occurring in patients with high viremia and persistent infection are the main source of new SARS-CoV-2 variants. |
Note: | Reproducció del document publicat a: https://doi.org/10.1080/22221751.2021.1969868 |
It is part of: | Emerging Microbes & Infections, 2021, vol. 10, num. 1, p. 1777-1789 |
URI: | http://hdl.handle.net/2445/192657 |
Related resource: | https://doi.org/10.1080/22221751.2021.1969868 |
ISSN: | 2222-1751 |
Appears in Collections: | Articles publicats en revistes (Genètica, Microbiologia i Estadística) |
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