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http://hdl.handle.net/2445/192910
Title: | Phosphorylation disrupts long-distance electron transport in cytochrome c |
Author: | Gomila, Alexandre M. J. Pérez-Mejías, Gonzalo Nin-Hill, Alba Guerra-Castellano, Alejandra Casas-Ferrer, Laura Ortiz Tescari, Sthefany Díaz Quintana, Antonio Samitier i Martí, Josep Rovira i Virgili, Carme De la Rosa, Miguel A. Díaz Moreno, Irene Gorostiza, Pau Giannotti, Marina I. Lagunas, Anna |
Keywords: | Química bioinorgànica Proteïnes Bioinorganic chemistry Proteins |
Issue Date: | 19-Nov-2022 |
Publisher: | Nature Publishing Group |
Abstract: | It has been recently shown that electron transfer between mitochondrial cytochrome c and the cytochrome c1 subunit of the cytochrome bc1 can proceed at long-distance through the aqueous solution. Cytochrome c is thought to adjust its activity by changing the affinity for its partners via Tyr48 phosphorylation, but it is unknown how it impacts the nanoscopic environment, interaction forces, and long-range electron transfer. Here, we constrain the orientation and separation between cytochrome c1 and cytochrome c or the phosphomimetic Y48pCMF cytochrome c, and deploy an array of single-molecule, bulk, and computational methods to investigate the molecular mechanism of electron transfer regulation by cytochrome c phosphorylation. We demonstrate that phosphorylation impairs long-range electron transfer, shortens the long-distance charge conduit between the partners, strengthens their interaction, and departs it from equilibrium. These results unveil a nanoscopic view of the interaction between redox protein partners in electron transport chains and its mechanisms of regulation. |
Note: | Reproducció del document publicat a: https://doi.org/10.1038/s41467-022-34809-1 |
It is part of: | Nature Communications, 2022, vol. 13, p. 7100 |
URI: | http://hdl.handle.net/2445/192910 |
Related resource: | https://doi.org/10.1038/s41467-022-34809-1 |
ISSN: | 2041-1723 |
Appears in Collections: | Articles publicats en revistes (Ciència dels Materials i Química Física) |
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