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http://hdl.handle.net/2445/194956
Title: | Image-Based Method to Quantify Decellularization of Tissue Sections |
Author: | Narciso, Maria Otero Díaz, Jorge Navajas Navarro, Daniel Farré Ventura, Ramon Almendros López, Isaac Gavara i Casas, Núria |
Keywords: | Teixits (Histologia) ADN Imatge corporal Microscòpia de fluorescència Processament d'imatges Divisió cel·lular Tissues DNA Body image Fluorescence microscopy Image processing Cell division |
Issue Date: | 1-Aug-2021 |
Publisher: | MDPI |
Abstract: | Tissue decellularization is typically assessed through absorbance-based DNA quantification after tissue digestion. This method has several disadvantages, namely its destructive nature and inadequacy in experimental situations where tissue is scarce. Here, we present an image processing algorithm for quantitative analysis of DNA content in (de)cellularized tissues as a faster, simpler and more comprehensive alternative. Our method uses local entropy measurements of a phase contrast image to create a mask, which is then applied to corresponding nuclei labelled (UV) images to extract average fluorescence intensities as an estimate of DNA content. The method can be used on native or decellularized tissue to quantify DNA content, thus allowing quantitative assessment of decellularization procedures. We confirm that our new method yields results in line with those obtained using the standard DNA quantification method and that it is successful for both lung and heart tissues. We are also able to accurately obtain a timeline of decreasing DNA content with increased incubation time with a decellularizing agent. Finally, the identified masks can also be applied to additional fluorescence images of immunostained proteins such as collagen or elastin, thus allowing further image-based tissue characterization |
Note: | Reproducció del document publicat a: https://doi.org/10.3390/ijms22168399 |
It is part of: | International Journal of Molecular Sciences, 2021, vol. 22, num. 16, p. 1-15 |
URI: | http://hdl.handle.net/2445/194956 |
Related resource: | https://doi.org/10.3390/ijms22168399 |
ISSN: | 1661-6596 |
Appears in Collections: | Articles publicats en revistes (Biomedicina) Articles publicats en revistes (Institut de Bioenginyeria de Catalunya (IBEC)) Articles publicats en revistes (IDIBAPS: Institut d'investigacions Biomèdiques August Pi i Sunyer) |
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