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http://hdl.handle.net/2445/196118
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DC Field | Value | Language |
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dc.contributor.author | Torre Tarazona, Humberto Erick de la | - |
dc.contributor.author | González Robles, Alba | - |
dc.contributor.author | Cascajero, Almudena | - |
dc.contributor.author | Jiménez, Paloma | - |
dc.contributor.author | Miró Meda, José M. | - |
dc.contributor.author | Sánchez Palomino, Sonsoles | - |
dc.contributor.author | Alcamí, José | - |
dc.contributor.author | Buzón, María J. | - |
dc.contributor.author | García Pérez, Javier | - |
dc.contributor.author | Hospital Clinic HIV Investigators | - |
dc.date.accessioned | 2023-03-28T17:21:25Z | - |
dc.date.available | 2023-03-28T17:21:25Z | - |
dc.date.issued | 2023-02-02 | - |
dc.identifier.issn | 0146-6615 | - |
dc.identifier.uri | http://hdl.handle.net/2445/196118 | - |
dc.description.abstract | The presence of neutralizing antibodies (NAbs) is a major correlate of protection for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. Thus, different in vitro pseudoviruses-based assays have been described to detect NAbs against SARS-CoV-2. However, the determination of NAbs against SARS-CoV-2 in people living with HIV (PLWH) through HIV-based pseudoparticles could be influenced by cross-neutralization activity or treatment, impeding accurate titration of NAbs. Two assays were compared using replication-defective HIV or VSV-based particles pseudotyped with SARS-CoV-2 spike to measure NAbs in COVID-19-recovered and COVID-19-naïve PLWH. The assay based on HIV-pseudoparticles displayed neutralization activity in all COVID-19-recovered PLWH with a median neutralizing titer 50 (NT50) of 1417.0 (interquartile range [IQR]: 450.3-3284.0), but also in 67% of COVID-19-naïve PLWH (NT50: 631.5, IQR: 16.0-1535.0). Regarding VSV-pseudoparticles system, no neutralization was observed in COVID-19-naïve PLWH as expected, whereas in comparison with HIV-pseudoparticles assay lower neutralization titers were measured in 75% COVID-19-recovered PLWH (NT50: 100.5; IQR: 20.5-1353.0). Treatment with integrase inhibitors was associated with inaccurate increase in neutralization titers when HIV-based pseudoparticles were used. IgG purification and consequent elimination of drugs from samples avoided the interference with retroviral cycle and corrected the lack of specificity observed in HIV-pseudotyped assay. This study shows methodological alternatives based on pseudoviruses systems to determine specific SARS-CoV-2 neutralization titers in PLWH. | - |
dc.format.extent | 8 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Wiley | - |
dc.relation.isformatof | Reproducció del document publicat a: https://doi.org/10.1002/jmv.28543 | - |
dc.relation.ispartof | Journal of Medical Virology, 2023, vol. 95, num. 2 | - |
dc.relation.uri | https://doi.org/10.1002/jmv.28543 | - |
dc.rights | cc-by (c) Torre Tarazona, Erick de la et al., 2023 | - |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es/ | * |
dc.source | Articles publicats en revistes (Medicina) | - |
dc.subject.classification | SARS-CoV-2 | - |
dc.subject.classification | Inhibidors de la integrasa | - |
dc.subject.classification | VIH (Virus) | - |
dc.subject.classification | Neutralitat | - |
dc.subject.classification | Persones seropositives | - |
dc.subject.other | SARS-CoV-2 | - |
dc.subject.other | Integrase inhibitors | - |
dc.subject.other | HIV (Viruses) | - |
dc.subject.other | Neutrality | - |
dc.subject.other | HIV-positive persons | - |
dc.title | Treatment with integrase inhibitors alters SARS-CoV-2 neutralization levels measured with HIV-based pseudotypes in people living with HIV | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.idgrec | 729611 | - |
dc.date.updated | 2023-03-28T17:21:25Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 36727646 | - |
Appears in Collections: | Articles publicats en revistes (Medicina) Articles publicats en revistes (IDIBAPS: Institut d'investigacions Biomèdiques August Pi i Sunyer) |
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729611.pdf | 1.21 MB | Adobe PDF | View/Open |
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