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http://hdl.handle.net/2445/197083
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DC Field | Value | Language |
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dc.contributor.author | Martínez-Mármol, Ramón | - |
dc.contributor.author | David, Miren | - |
dc.contributor.author | Sanches, Rosario | - |
dc.contributor.author | Roura-Ferrer, Meritxell | - |
dc.contributor.author | Villalonga, Núria | - |
dc.contributor.author | Sorianello, Eleonora | - |
dc.contributor.author | Webb, Susan M. | - |
dc.contributor.author | Zorzano Olarte, Antonio | - |
dc.contributor.author | Gumà i Garcia, Anna Maria | - |
dc.contributor.author | Valenzuela, Carmen | - |
dc.contributor.author | Felipe Campo, Antonio | - |
dc.date.accessioned | 2023-04-21T07:56:28Z | - |
dc.date.available | 2023-04-21T07:56:28Z | - |
dc.date.issued | 2007-12-01 | - |
dc.identifier.issn | 0008-6363 | - |
dc.identifier.uri | http://hdl.handle.net/2445/197083 | - |
dc.description.abstract | Objective: Cellular cardiomyoplasty using skeletal myoblasts is a promising therapy for myocardial infarct repair. Once transplanted, myoblasts grow, differentiate and adapt their electrophysiological properties towards more cardiac-like phenotypes. Voltage-dependent Na + channels (Na v ) are the main proteins involved in the propagation of the cardiac action potential, and their phenotype affects cardiac performance. Therefore, we examined the expression of Na v during proliferation and differentiation in skeletal myocytes. Methods and results: We used the rat neonatal skeletal myocyte cell line L6E9. Proliferation of L6E9 cells induced Na v 1.4 and Na v 1.5, although neither protein has an apparent role in cell growth. During myogenesis, Na v1.5 was largely induced. Electrophysiological and pharmacological properties, as well as mRNA expression, indicate that cardiac-type Na v1.5 accounts for almost 90% of the Na + current in myotubes. Unlike in proliferation, this protein plays a pivotal role in myogenesis. The adoption of a cardiac-like phenotype is further supported by the increase in Nav 1.5 colocalization in caveolae. Finally, we demonstrate that the treatment of myoblasts with neuregulin further increased Na v 1.5 in skeletal myocytes. Conclusion: Our results indicate that skeletal myotubes adopt a cardiac-like phenotype in cell culture conditions and that the expression of Na v1.5 acts as an underlying molecular mechanism. | - |
dc.format.extent | 12 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Oxford University Press | - |
dc.relation.isformatof | Versió postprint del document publicat a: https://doi.org/10.1016/j.cardiores.2007.08.009 | - |
dc.relation.ispartof | Cardiovascular Research, 2007, vol. 76, num. 3, p. 430-441 | - |
dc.relation.uri | https://doi.org/10.1016/j.cardiores.2007.08.009 | - |
dc.rights | (c) European Society of Cardiology, 2007 | - |
dc.source | Articles publicats en revistes (Bioquímica i Biomedicina Molecular) | - |
dc.subject.classification | Canals iònics | - |
dc.subject.classification | Miogènesi | - |
dc.subject.classification | Malalties del cor | - |
dc.subject.classification | Biologia del desenvolupament | - |
dc.subject.other | Ion channels | - |
dc.subject.other | Myogenesis | - |
dc.subject.other | Heart diseases | - |
dc.subject.other | Developmental biology | - |
dc.title | Voltage-dependent Na+ channel phenotype changes in myoblasts. Consequences for cardiac repair | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/acceptedVersion | - |
dc.identifier.idgrec | 553982 | - |
dc.date.updated | 2023-04-21T07:56:28Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
Appears in Collections: | Articles publicats en revistes (Bioquímica i Biomedicina Molecular) |
Files in This Item:
File | Description | Size | Format | |
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553982.pdf | 1.88 MB | Adobe PDF | View/Open |
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