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Title: | Monitoring dexamethasone skin biodistribution with ex vivo MALDI-TOF mass spectrometry imaging and confocal Raman microscopy |
Author: | Pena Rodríguez, Eloy García Berrocoso, Teresa Vázquez Fernández, Ezequiel Otero Espinar, Francisco J. Abian, Joaquin Fernández Campos, Francisco |
Keywords: | Pell Espectrometria de masses Skin Mass spectrometry |
Issue Date: | 1-Apr-2023 |
Publisher: | Elsevier |
Abstract: | Two of the most promising techniques in terms of ex vivo skin imaging and quantifying are confocal Raman microscopy and MALDI-TOF mass spectrometry imaging (MALDI-TOF MSI). Both techniques were set up, and the semiquantitative skin biodistribution of previously developed dexamethasone (DEX) loaded lipomers was compared using Benzalkonium chloride (BAK) as a tracer of the nanoparticles. In MALDI-TOF MSI, DEX was derivatised with GirT (DEX-GirT) and the semiquantitative biodistribution of both DEX-GirT and BAK was successfully obtained. The amount of DEX measured by confocal Raman microscopy was higher than that measured by MALDI-TOF MSI, but MALDI-TOF MSI proved to be a more suitable technique for tracing BAK. An absorption-promoting tendency of DEX loaded in lipomers versus a free-DEX solution was observed in confocal Raman microscopy. The higher spatial resolution of confocal Raman microscopy (350 nm) with respect to MALDI-TOF MSI (50 mu m) allowed to observe specific skin structures like hair follicles. Nevertheless, the faster sampling rate of MALDI-TOF-MSI, permitted the analysis of larger tissue regions. In conclusion, both techniques allowed to simultaneously analyze semiquantitative data together with qualitative images of biodistribution, which is a very helpful tool when designing nanoparticles that accumulate in specific anatomical regions. |
Note: | Reproducció del document publicat a: https://doi.org/10.1016/j.ijpharm.2023.122808 |
It is part of: | International Journal of Pharmaceutics, 2023, vol. 636 |
URI: | http://hdl.handle.net/2445/197328 |
Related resource: | https://doi.org/10.1016/j.ijpharm.2023.122808 |
ISSN: | 1873-3476 |
Appears in Collections: | Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) |
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