Please use this identifier to cite or link to this item:
http://hdl.handle.net/2445/206968
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Yeramian, Andrée | - |
dc.contributor.author | Martin, Lorena | - |
dc.contributor.author | Arpa, Luis | - |
dc.contributor.author | Bertran, Joan, 1964- | - |
dc.contributor.author | Soler Prat, Concepció | - |
dc.contributor.author | McLeod, Carol | - |
dc.contributor.author | Modolell, Manuel | - |
dc.contributor.author | Palacín Prieto, Manuel | - |
dc.contributor.author | Lloberas Cavero, Jorge | - |
dc.contributor.author | Celada Cotarelo, Antonio | - |
dc.date.accessioned | 2024-02-01T14:38:34Z | - |
dc.date.available | 2024-02-01T14:38:34Z | - |
dc.date.issued | 2006-06-01 | - |
dc.identifier.issn | 0014-2980 | - |
dc.identifier.uri | http://hdl.handle.net/2445/206968 | - |
dc.description.abstract | In murine macrophages, as a result of arginine catabolism during activation, citruline isproduced under the effect of IFN-c and LPS, and ornithine and polyamines by IL-4 and IL-10. For proliferation, arginine is required from the extracellular medium and is used for protein synthesis. During activation, most arginine (>95% in 6 h) was metabolized, while under proliferation only half was incorporated into proteins. Under basal conditions, this amino acid was preferentially transported by y+L activity. During activation, arginine transport increased drastically (4–5-fold) through y+ cationic amino acid transporter (CAT) activity. By contrast, M-CSF induced only a modest increase in uptake (0.5-fold). The increase in arginine transport during activation, but not proliferation, was mediated by the SLC7A2/Cat2 gene. SLC7A1/Cat1 is constitutively expressed, and is not modified by proliferating or activating agents.M-CSF-dependent proliferation was not affected in the macrophages of SLC7A2 knockout mice; however, these cells showed a drastic reduction in the production of citruline or ornithine and polyamines during activation. The data show that a large increase in a specific transport system (CAT2) is necessary for activation-induced arginine metabolism, while arginine is in excess for the requirements of proliferation and a modest increase in transport occurs. | - |
dc.format.extent | 11 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Wiley-VCH | - |
dc.relation.isformatof | Versió postprint del document publicat a: https://doi.org/https://doi.org/10.1002/eji.200535694 | - |
dc.relation.ispartof | European Journal of Immunology, 2006, vol. 36, num.6, p. 1516-1526 | - |
dc.relation.uri | https://doi.org/https://doi.org/10.1002/eji.200535694 | - |
dc.rights | (c) Wiley-VCH, 2006 | - |
dc.source | Articles publicats en revistes (Patologia i Terapèutica Experimental) | - |
dc.subject.classification | Macròfags | - |
dc.subject.classification | Proliferació cel·lular | - |
dc.subject.other | Macrophages | - |
dc.subject.other | Cell proliferation | - |
dc.title | Macrophages require distinct arginine catabolism and transport systems for proliferation and for activation. | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/acceptedVersion | - |
dc.identifier.idgrec | 570043 | - |
dc.date.updated | 2024-02-01T14:38:34Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
Appears in Collections: | Articles publicats en revistes (Patologia i Terapèutica Experimental) |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
124278.pdf | 1.19 MB | Adobe PDF | View/Open |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.