Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/21494
Title: E2F1 regulates cellular growth by mTOR signaling
Author: Real, Sebastian
Meo-Evoli, Nathalie
Espada, Lilia
Tauler Girona, Albert
Keywords: Regulació cel·lular
Biologia molecular
Cellular control mechanisms
Molecular biology
Issue Date: 24-Jan-2011
Publisher: PLoS
Abstract: During cell proliferation, growth must occur to maintain homeostatic cell size. Here we show that E2F1 is capable of inducing growth by regulating mTORC1 activity. The activation of cell growth and mTORC1 by E2F1 is dependent on both E2F1's ability to bind DNA and to regulate gene transcription, demonstrating that a gene induction expression program is required in this process. Unlike E2F1, E2F3 is unable to activate mTORC1, suggesting that growth activity could be restricted to individual E2F members. The effect of E2F1 on the activation of mTORC1 does not depend on Akt. Furthermore, over-expression of TSC2 does not interfere with the effect of E2F1, indicating that the E2F1-induced signal pathway can compensate for the inhibitory effect of TSC2 on Rheb. Immunolocalization studies demonstrate that E2F1 induces the translocation of mTORC1 to the late endosome vesicles, in a mechanism dependent of leucine. E2F1 and leucine, or insulin, together affect the activation of S6K stronger than alone suggesting that they are complementary in activating the signal pathway. From these studies, E2F1 emerges as a key protein that integrates cell division and growth, both of which are essential for cell proliferation.
Note: Reproducció del document publicat a: dx.doi.org/10.1371/journal.pone.0016163
It is part of: PLoS ONE, 2011, 6(1): e16163
URI: http://hdl.handle.net/2445/21494
ISSN: 1932-6203
Appears in Collections:Articles publicats en revistes (Bioquímica i Biomedicina Molecular)

Files in This Item:
File Description SizeFormat 
580993.pdf1.92 MBAdobe PDFView/Open


This item is licensed under a Creative Commons License Creative Commons