Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/21743
Title: Indirect DNA Readout by an H-NS Related Protein: Structure of the DNA Complex of the C-Terminal Domain of Ler
Author: Cordeiro, Tiago N.
Schmidt, Holger
Madrid Xufré, Cristina
Juárez Giménez, Antonio
Bernadó Peretó, Pau
Griesinger, Christian
García, Jesús
Pons Vallès, Miquel
Keywords: ADN
Expressió gènica
Transcripció genètica
DNA
Gene expression
Genetic transcription
Issue Date: Nov-2011
Publisher: PLoS
Abstract: Ler, a member of the H-NS protein family, is the master regulator of the LEE pathogenicity island in virulent Escherichia coli strains. Here, we determined the structure of a complex between the DNA-binding domain of Ler (CT-Ler) and a 15-mer DNA duplex. CT-Ler recognizes a preexisting structural pattern in the DNA minor groove formed by two consecutive regions which are narrower and wider, respectively, compared with standard B-DNA. The compressed region, associated with an AT-tract, is sensed by the side chain of Arg90, whose mutation abolishes the capacity of Ler to bind DNA. The expanded groove allows the approach of the loop in which Arg90 is located. This is the first report of an experimental structure of a DNA complex that includes a protein belonging to the H-NS family. The indirect readout mechanism not only explains the capacity of H-NS and other H-NS family members to modulate the expression of a large number of genes but also the origin of the specificity displayed by Ler. Our results point to a general mechanism by which horizontally acquired genes may be specifically recognized by members of the H-NS family.
Note: Reproducció del document publicat a: http://dx.doi.org/10.1371/journal.ppat.1002380
It is part of: PLoS Pathogens, 2011, 7(11): e1002380
Related resource: http://dx.doi.org/10.1371/journal.ppat.1002380
URI: http://hdl.handle.net/2445/21743
ISSN: 1553-7366
Appears in Collections:Articles publicats en revistes (Genètica, Microbiologia i Estadística)
Articles publicats en revistes (Química Inorgànica i Orgànica)
Publicacions de projectes de recerca finançats per la UE

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