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http://hdl.handle.net/2445/24655
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DC Field | Value | Language |
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dc.contributor.author | Sarri Plans, Elisabet | cat |
dc.contributor.author | Martínez Rodríguez, Susana Eva | cat |
dc.contributor.author | Jiménez, Nuria | cat |
dc.contributor.author | Claro Izaguirre, Enrique | cat |
dc.contributor.author | Mérida, Isabel | cat |
dc.contributor.author | Burger, Koert N. J. | cat |
dc.contributor.author | Egea Guri, Gustavo | cat |
dc.contributor.author | Fernández-Ulibarri, Inés | - |
dc.contributor.author | Vilella, Montserrat | - |
dc.contributor.author | Lázaro Diégez, Francisco | - |
dc.date.accessioned | 2012-04-27T09:52:34Z | - |
dc.date.available | 2012-04-27T09:52:34Z | - |
dc.date.issued | 2007 | - |
dc.identifier.issn | 1059-1524 | - |
dc.identifier.uri | http://hdl.handle.net/2445/24655 | - |
dc.description.abstract | Diacylglycerol is necessary for trans-Golgi network (TGN) to cell surface transport, but its functional relevance in the early secretory pathway is unclear. Although depletion of diacylglycerol did not affect ER-to-Golgi transport, it led to a redistribution of the KDEL receptor to the Golgi, indicating that Golgi-to-ER transport was perturbed. Electron microscopy revealed an accumulation of COPI-coated membrane profiles close to the Golgi cisternae. Electron tomography showed that the majority of these membrane profiles originate from coated buds, indicating a block in membrane fission. Under these conditions the Golgi-associated pool of ARFGAP1 was reduced, but there was no effect on the binding of coatomer or the membrane fission protein CtBP3/BARS to the Golgi. The addition of 1,2-dioctanoyl-sn-glycerol or the diacylglycerol analogue phorbol 12,13-dibutyrate reversed the effects of endogenous diacylglycerol depletion. Our findings implicate diacylglycerol in the retrograde transport of proteins from Golgi to the ER and suggest that it plays a critical role at a late stage of COPI vesicle formation. | eng |
dc.format.extent | 14 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | eng |
dc.publisher | American Society for Cell Biology | - |
dc.relation.isformatof | Reproducció del document publicat a: http://dx.doi.org/10.1091/mbc.E07-04-0334 | - |
dc.relation.ispartof | Molecular Biology of the Cell, 2007, vol. 18, núm. 9, p. 3250-3263 | - |
dc.rights | cc-by-nc-sa (c) Fernández-Ulibarri et al., 2007 | - |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/3.0/ | - |
dc.source | Articles publicats en revistes (Biomedicina) | - |
dc.subject.classification | Aparell de Golgi | cat |
dc.subject.classification | Reticle endoplasmàtic | cat |
dc.subject.classification | Proteïnes | cat |
dc.subject.classification | Citologia | cat |
dc.subject.classification | Transport biològic | cat |
dc.subject.other | Golgi apparatus | eng |
dc.subject.other | Endoplasmic reticulum | eng |
dc.subject.other | Proteins | eng |
dc.subject.other | Cytology | eng |
dc.subject.other | Biological transport | eng |
dc.title | Diacylglycerol is required for the formation of COPI vesicles in the Golgi-to-ER transport pathway | eng |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.doi | http://dx.doi.org/10.1091/mbc.E07-04-0334 | - |
dc.identifier.idgrec | 552867 | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 17567948 | - |
Appears in Collections: | Articles publicats en revistes (Biomedicina) |
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552867.pdf | 4.48 MB | Adobe PDF | View/Open |
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