Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/24656
Title: Vacuole membrane protein 1 is an endoplasmic reticulum protein required for organelle biogenesis, protein secretion, and development
Author: Calvo-Garrido, Javier
Carilla-Latorre, Sergio
Lázaro Diégez, Francisco
Egea Guri, Gustavo
Keywords: Proteïnes de membrana
Citologia
Citogenètica
Reticle endoplasmàtic
Membrane proteins
Cytology
Cytogenetics
Endoplasmic reticulum
Issue Date: 2008
Publisher: American Society for Cell Biology
Abstract: Vacuole membrane protein 1 (Vmp1) is membrane protein of unknown molecular function that has been associated with pancreatitis and cancer. The social amoeba Dictyostelium discoideum has a vmp1-related gene that we identified previously in a functional genomic study. Loss-of-function of this gene leads to a severe phenotype that compromises Dictyostelium growth and development. The expression of mammalian Vmp1 in a vmp1 Dictyostelium mutant complemented the phenotype, suggesting a functional conservation of the protein among evolutionarily distant species and highlights Dictyostelium as a valid experimental system to address the function of this gene. Dictyostelium Vmp1 is an endoplasmic reticulum protein necessary for the integrity of this organelle. Cells deficient in Vmp1 display pleiotropic defects in the secretory pathway and organelle biogenesis. The contractile vacuole, which is necessary to survive under hypoosmotic conditions, is not functional in the mutant. The structure of the Golgi apparatus, the function of the endocytic pathway and conventional protein secretion are also affected in these cells. Transmission electron microscopy of vmp1 cells showed the accumulation of autophagic features that suggests a role of Vmp1 in macroautophagy. In addition to these defects observed at the vegetative stage, the onset of multicellular development and early developmental gene expression are also compromised.
Note: Reproducció del document publicat a: http://dx.doi.org/10.1091/mbc.E08-01-0075
It is part of: Molecular Biology of the Cell, 2008, vol. 19, núm. 8, p. 3442-3453
Related resource: http://dx.doi.org/10.1091/mbc.E08-01-0075
URI: http://hdl.handle.net/2445/24656
ISSN: 1059-1524
Appears in Collections:Articles publicats en revistes (Biomedicina)

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