Please use this identifier to cite or link to this item:
|Title:||Assembly and disassembly of the Golgi Complex: two prodesses arranged in a cis to trans direction|
Vilaró, Senén, 1956-2005
Sandoval, Ignacio V.
Aparell de Golgi
|Publisher:||Rockefeller University Press|
|Abstract:||We have studied the disassembly and assembly of two morphologically and functionally distinct parts of the Golgi complex, the cis/middle and trans cisterna/trans network compartments. For this purpose we have followed the redistribution of three cis/middle- (GMPc-1, GMPc-2, MG 160) and two trans- (GMPt-1 and GMPt-2) Golgi membrane proteins during and after treatment of normal rat kidney (NRK) cells with brefeldin A (BFA). BFA induced complete disassembly of the cis/middle- and trans-Golgi complex and translocation of GMPc and GMPt to the ER. Cells treated for short times (3 min) with BFA showed extensive disorganization of both cis/middle- and trans-Golgi complexes. However, complete disorganization of the trans part required much longer incubations with the drug. Upon removal of BFA the Golgi complex was reassembled by a process consisting of three steps: (a) exist of cis/middle proteins from the ER and their accumulation into vesicular structures scattered throughout the cytoplasm; (b) gradual relocation and accumulation of the trans proteins in the vesicles containing the cis/middle proteins; and (c) assembly of the cisternae, and reconstruction of the Golgi complex within an area located in the vicinity of the centrosome from which the ER was excluded. Reconstruction of the cis/middle-Golgi complex occurred under temperature conditions inhibitory of the reorganization of the trans-Golgi complex, and was dependent on microtubules. Reconstruction of the trans-Golgi complex, disrupted with nocodazole after selective fusion of the cis/middle-Golgi complex with the ER, occurred after the release of cis/middle-Golgi proteins from the ER and the assembly of the cis/middle cisternae.|
|Note:||Reproducció digital del document publicat a: http://dx.doi.org/10.1083/jcb.116.1.69|
|It is part of:||Journal of Cell Biology, 1992, vol. 116, núm. 1, p. 69-83|
|Appears in Collections:||Articles publicats en revistes (Biologia Cel·lular, Fisiologia i Immunologia)|
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.