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http://hdl.handle.net/2445/25214
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DC Field | Value | Language |
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dc.contributor.author | Serrador, Juan M. | cat |
dc.contributor.author | Alonso Lebrero, José L. | cat |
dc.contributor.author | Pozo, Miguel A. del | cat |
dc.contributor.author | Furthmayr, Heinz | cat |
dc.contributor.author | Schwartz-Albiez, Reinhard | cat |
dc.contributor.author | Calvo, Javier | cat |
dc.contributor.author | Lozano Soto, Francisco | cat |
dc.contributor.author | Sánchez-Madrid, Francisco | cat |
dc.date.accessioned | 2012-05-09T10:01:15Z | - |
dc.date.available | 2012-05-09T10:01:15Z | - |
dc.date.issued | 1997-09-22 | - |
dc.identifier.issn | 0021-9525 | - |
dc.identifier.uri | http://hdl.handle.net/2445/25214 | - |
dc.description.abstract | During activation, T lymphocytes become motile cells, switching from a spherical to a polarized shape. Chemokines and other chemotactic cytokines induce lymphocyte polarization with the formation of a uropod in the rear pole, where the adhesion receptors intercellular adhesion molecule-1 (ICAM-1), ICAM-3, and CD44 redistribute. We have investigated membrane-cytoskeleton interactions that play a key role in the redistribution of adhesion receptors to the uropod. Immunofluorescence analysis showed that the ERM proteins radixin and moesin localized to the uropod of human T lymphoblasts treated with the chemokine RANTES (regulated on activation, normal T cell expressed, and secreted), a polarization-inducing agent; radixin colocalized with arrays of myosin II at the neck of the uropods, whereas moesin decorated the most distal part of the uropod and colocalized with ICAM-1, ICAM-3, and CD44 molecules. Two other cytoskeletal proteins, ß-actin and ¿-tubulin, clustered at the cell leading edge and uropod, respectively, of polarized lymphocytes. Biochemical analysis showed that moesin coimmunoprecipitates with ICAM-3 in T lymphoblasts stimulated with either RANTES or the polarization- inducing anti-ICAM-3 HP2/19 mAb, as well as in the constitutively polarized T cell line HSB-2. In addition, moesin is associated with CD44, but not with ICAM-1, in polarized T lymphocytes. A correlation between the degree of moesin-ICAM-3 interaction and cell polarization was found as determined by immunofluorescence and immunoprecipitation analysis done in parallel. The moesin-ICAM-3 interaction was specifically mediated by the cytoplasmic domain of ICAM-3 as revealed by precipitation of moesin with a GST fusion protein containing the ICAM-3 cytoplasmic tail from metabolically labeled Jurkat T cell lysates. The interaction of moesin with ICAM-3 was greatly diminished when RANTES-stimulated T lymphoblasts were pretreated with the myosin-disrupting drug butanedione monoxime, which prevents lymphocyte polarization. Altogether, these data indicate that moesin interacts with ICAM-3 and CD44 adhesion molecules in uropods of polarized T cells; these data also suggest that these interactions participate in the formation of links between membrane receptors and the cytoskeleton, thereby regulating morphological changes during cell locomotion. | eng |
dc.format.extent | 15 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | eng |
dc.publisher | Rockefeller University Press | - |
dc.relation.isformatof | Reproducció digital del document publicat a: http://dx.doi.org/10.1083/jcb.138.6.1409 | - |
dc.relation.ispartof | Journal of Cell Biology, 1997, vol. 138, núm. 6, p. 1409-1423 | - |
dc.relation.uri | http://dx.doi.org/10.1083/jcb.138.6.1409 | - |
dc.rights | (c) Rockefeller University Press, 1997 | - |
dc.source | Articles publicats en revistes (Biomedicina) | - |
dc.subject.classification | Interacció cel·lular | - |
dc.subject.classification | Cèl·lules T | cat |
dc.subject.other | Cell interaction | - |
dc.title | Moesin interacts with the cytoplasmic region of intercellular adhesion molecule-3 and is redistributed to the uropod of T Lymphocytes during cell polarization | eng |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.idgrec | 530389 | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 9298994 | - |
Appears in Collections: | Articles publicats en revistes (Biomedicina) |
Files in This Item:
File | Description | Size | Format | |
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530389.pdf | 9.39 MB | Adobe PDF | View/Open |
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