Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/25226
Title: MYADM regulates Rac1 targeting to ordered membranes required for cell spreading and migration
Author: Aranda, Juan Francisco
Reglero-Real, Natalia
Kremer, Leonor
Marcos-Ramiro, Beatriz
Ruiz-Sáenz, Ana
Calvo Ademuz, Maria
Enrich Bastús, Carles
Correas, Isabel
Millán, Jaime
Alonso, Miguel Ángel
Keywords: Membranes (Biologia)
Citologia
Cytology
Membranes (Biology)
Issue Date: 8-Feb-2011
Publisher: American Society for Cell Biology
Abstract: Membrane organization into condensed domains or rafts provides molecular platforms for selective recruitment of proteins. Cell migration is a general process that requires spatiotemporal targeting of Rac1 to membrane rafts. The protein machinery responsible for making rafts competent to recruit Rac1 remains elusive. Some members of the MAL family of proteins are involved in specialized processes dependent on this type of membrane. Because condensed membrane domains are a general feature of the plasma membrane of all mammalian cells, we hypothesized that MAL family members with ubiquitous expression and plasma membrane distribution could be involved in the organization of membranes for cell migration. We show that myeloid-associated differentiation marker (MYADM), a protein with unique features within the MAL family, colocalizes with Rac1 in membrane protrusions at the cell surface and distributes in condensed membranes. MYADM knockdown (KD) cells had altered membrane condensation and showed deficient incorporation of Rac1 to membrane raft fractions and, similar to Rac1 KD cells, exhibited reduced cell spreading and migration. Results of rescue-of-function experiments by expression of MYADM or active Rac1L61 in cells knocked down for Rac1 or MYADM, respectively, are consistent with the idea that MYADM and Rac1 act on parallel pathways that lead to similar functional outcomes.
Note: Reproducció del document publicat a: http://dx.doi.org/10.1091/mbc.E10-11-0910
It is part of: Molecular Biology of the Cell, 2011, vol. 22, núm. 8, p. 1252-1262
Related resource: http://dx.doi.org/10.1091/mbc.E10-11-0910
URI: http://hdl.handle.net/2445/25226
ISSN: 1059-1524
Appears in Collections:Articles publicats en revistes (Biomedicina)

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