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|Title:||Cholesterol and Fatty Acids Regulate Dynamic Caveolin Trafficking through the Golgi Complex and between the Cell Surface and Lipid Bodies|
|Author:||Pol i Sorolla, Albert|
Fernandez, Manuel A.
Enrich Bastús, Carles
Parton, Robert G.
Proteïnes de membrana
Aparell de Golgi
|Publisher:||American Society for Cell Biology|
|Abstract:||Caveolins are a crucial component of plasma membrane (PM) caveolae but have also been localized to intracellular compartments, including the Golgi complex and lipid bodies. Mutant caveolins associated with human disease show aberrant trafficking to the PM and Golgi accumulation. We now show that the Golgi pool of mainly newly synthesized protein is detergent-soluble and predominantly in a monomeric state, in contrast to the surface pool. Caveolin at the PM is not recognized by specific caveolin antibodies unless PM cholesterol is depleted. Exit from the Golgi complex of wild-type caveolin-1 or -3, but not vesicular stomatitis virus-G protein, is modulated by changing cellular cholesterol levels. In contrast, a muscular dystrophy-associated mutant of caveolin-3, Cav3P104L, showed increased accumulation in the Golgi complex upon cholesterol treatment. In addition, we demonstrate that in response to fatty acid treatment caveolin can follow a previously undescribed pathway from the PM to lipid bodies and can move from lipid bodies to the PM in response to removal of fatty acids. The results suggest that cholesterol is a rate-limiting component for caveolin trafficking. Changes in caveolin flux through the exocytic pathway can therefore be an indicator of cellular cholesterol and fatty acid levels.|
|Note:||Reproducció del document publicat a: http://dx.doi.org/10.1091/mbc.E04-08-0737|
|It is part of:||Molecular Biology of the Cell, 2005, vol. 16, núm. 4, p. 2091-2105|
|Appears in Collections:||Articles publicats en revistes (Biomedicina)|
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