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Title: | The gammaTuRC revisited: A comparative analysis of interphase and mitotic human gammaTuRC redefines the set of core components and identifies the novel subunit GCP8 |
Author: | Teixidó i Travesa, Neus Villén, Judit Lacasa Salavert, Cristina Bertran, Maria Teresa Archinti, Marco Gygi, Steven P. Caelles Franch, Carme Roig Amorós, Joan Lüders, Jens |
Keywords: | Citologia Biologia del desenvolupament Cytology Developmental biology |
Issue Date: | 13-Sep-2010 |
Publisher: | American Society for Cell Biology |
Abstract: | The γ-tubulin complex is a multi-subunit protein complex that nucleates microtubule polymerization. γ-Tubulin complexes are present in all eukaryotes, but size and subunit composition vary. In Drosophila, Xenopus, and humans large γ-tubulin ring complexes (γTuRCs) have been described, which have a characteristic open ring-shaped structure and are composed of a similar set of subunits, named γ-tubulin, GCPs 2-6, and GCP-WD in humans. Despite the identification of these proteins, γTuRC function and regulation remain poorly understood. Here we establish a new method for the purification of native human γTuRC. Using mass spectrometry of whole protein mixtures we compared the composition of γTuRCs from nonsynchronized and mitotic human cells. Based on our analysis we can define core subunits as well as more transient interactors such as the augmin complex, which associates specifically with mitotic γTuRCs. We also identified GCP8/MOZART2 as a novel core subunit that is present in both interphase and mitotic γTuRCs. GCP8 depletion does not affect γTuRC assembly but interferes with γTuRC recruitment and microtubule nucleation at interphase centrosomes without disrupting general centrosome structure. GCP8-depleted cells do not display any obvious mitotic defects, suggesting that GCP8 specifically affects the organization of the interphase microtubule network. |
Note: | Reproducció del document publicat a: http://dx.doi.org/10.1091/mbc.E10-05-0408 |
It is part of: | Molecular Biology of the Cell, 2010, vol. 21, núm. 22, p. 3963-3972 |
URI: | http://hdl.handle.net/2445/25262 |
Related resource: | http://dx.doi.org/10.1091/mbc.E10-05-0408 |
ISSN: | 1059-1524 |
Appears in Collections: | Articles publicats en revistes (Bioquímica i Biomedicina Molecular) Publicacions de projectes de recerca finançats per la UE |
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