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http://hdl.handle.net/2445/25622
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DC Field | Value | Language |
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dc.contributor.author | Alcaraz Casademunt, Jordi | cat |
dc.contributor.author | Buscemi Estefanell, Lara | cat |
dc.contributor.author | Grabulosa Descals, Mireia | cat |
dc.contributor.author | Trepat Guixer, Xavier | cat |
dc.contributor.author | Fabry, Ben | cat |
dc.contributor.author | Farré Ventura, Ramon | cat |
dc.contributor.author | Navajas Navarro, Daniel | cat |
dc.date | Lung epithelial cells are subjected to large cyclic forces from breathing. However, their response to dynamic stresses is poorly defined. We measured the complex shear modulus ( G * ( ω )) of human alveolar (A549) and bronchial (BEAS-2B) epithelial cells over three frequency decades (0.1–100Hz) and at different loading forces (0.1–0.9nN) with atomic force microscopy. G * ( ω ) was computed by correcting force-indentation oscillatory data for the tip-cell contact geometry and for the hydrodynamic viscous drag. Both cell types displayed similar viscoelastic properties. The storage modulus G ′( ω ) increased with frequency following a power law with exponent ∼0.2. The loss modulus G ″( ω ) was ∼2/3 lower and increased similarly to G ′( ω ) up to ∼10Hz, but exhibited a steeper rise at higher frequencies. The cells showed a weak force dependence of G ′( ω ) and G ″( ω ). G * ( ω ) conformed to the power-law model with a structural damping coefficient of ∼0.3, indicating a coupling of elastic and dissipative processes within the cell. Power-law behavior implies a continuum distribution of stress relaxation time constants. This complex dynamics is consistent with the rheology of soft glassy materials close to a glass transition, thereby suggesting that structural disorder and metastability may be fundamental features of cell architecture. | - |
dc.date.accessioned | 2012-05-14T14:12:23Z | - |
dc.date.available | 2012-05-14T14:12:23Z | - |
dc.date.issued | 2003 | - |
dc.identifier.issn | 0006-3495 | - |
dc.identifier.uri | http://hdl.handle.net/2445/25622 | - |
dc.description.abstract | Lung epithelial cells are subjected to large cyclic forces from breathing. However, their response to dynamic stresses is poorly defined. We measured the complex shear modulus (G*(ω)) of human alveolar (A549) and bronchial (BEAS-2B) epithelial cells over three frequency decades (0.1–100 Hz) and at different loading forces (0.1–0.9 nN) with atomic force microscopy. G*(ω) was computed by correcting force-indentation oscillatory data for the tip-cell contact geometry and for the hydrodynamic viscous drag. Both cell types displayed similar viscoelastic properties. The storage modulus G′(ω) increased with frequency following a power law with exponent ∼0.2. The loss modulus G″(ω) was ∼2/3 lower and increased similarly to G′(ω) up to ∼10 Hz, but exhibited a steeper rise at higher frequencies. The cells showed a weak force dependence of G′(ω) and G″(ω). G*(ω) conformed to the power-law model with a structural damping coefficient of ∼0.3, indicating a coupling of elastic and dissipative processes within the cell. Power-law behavior implies a continuum distribution of stress relaxation time constants. This complex dynamics is consistent with the rheology of soft glassy materials close to a glass transition, thereby suggesting that structural disorder and metastability may be fundamental features of cell architecture. | - |
dc.format.extent | 9 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | eng |
dc.publisher | Biophysical Society | - |
dc.relation.isformatof | Reproducció del document publicat a: http://dx.doi.org/10.1016/S0006-3495(03)75014-0 | - |
dc.relation.ispartof | Biophysical Journal, 2003, vol. 84, num. 3, p. 2071-2079 | - |
dc.relation.uri | http://dx.doi.org/10.1016/S0006-3495(03)75014-0 | - |
dc.rights | (c) Biophysical Society, 2003 | - |
dc.source | Articles publicats en revistes (Ciències Fisiològiques) | - |
dc.subject.classification | Microscòpia de força atòmica | cat |
dc.subject.classification | Membrana mucosa | cat |
dc.subject.classification | Reologia (Biologia) | cat |
dc.subject.other | Atomic force microscopy | eng |
dc.subject.other | Mucous membrane | eng |
dc.subject.other | Rheology (Biology) | eng |
dc.title | Microrheology of human lung epithelial cells measured by atomic force microscopy | eng |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.idgrec | 504995 | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | ca |
dc.identifier.pmid | 12609908 | - |
Appears in Collections: | Articles publicats en revistes (Ciències Fisiològiques) |
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504995.pdf | 145.79 kB | Adobe PDF | View/Open |
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