Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/25802
Title: Micropatterning of single endothelial cell shape reveals a tight coupling between nuclear volume in G1 and proliferation
Author: Roca-Cusachs Soulere, Pere
Alcaraz Casademunt, Jordi
Sunyer, Raimon
Samitier i Martí, Josep
Farré Ventura, Ramon
Navajas Navarro, Daniel
Keywords: Cèl·lules epitelials
Regulació cel·lular
Cicle cel·lular
Epithelial cells
Cellular control mechanisms
Cell cycle
Issue Date: 2008
Publisher: Biophysical Society
Abstract: Shape-dependent local differentials in cell proliferation are considered to be a major driving mechanism of structuring processes in vivo, such as embryogenesis, wound healing, and angiogenesis. However, the specific biophysical signaling by which changes in cell shape contribute to cell cycle regulation remains poorly understood. Here, we describe our study of the roles of nuclear volume and cytoskeletal mechanics in mediating shape control of proliferation in single endothelial cells. Micropatterned adhesive islands were used to independently control cell spreading and elongation. We show that, irrespective of elongation, nuclear volume and apparent chromatin decondensation of cells in G1 systematically increased with cell spreading and highly correlated with DNA synthesis (percent of cells in the S phase). In contrast, cell elongation dramatically affected the organization of the actin cytoskeleton, markedly reduced both cytoskeletal stiffness (measured dorsally with atomic force microscopy) and contractility (measured ventrally with traction microscopy), and increased mechanical anisotropy, without affecting either DNA synthesis or nuclear volume. Our results reveal that the nuclear volume in G1 is predictive of the proliferative status of single endothelial cells within a population, whereas cell stiffness and contractility are not. These findings show that the effects of cell mechanics in shape control of proliferation are far more complex than a linear or straightforward relationship. Our data are consistent with a mechanism by which spreading of cells in G1 partially enhances proliferation by inducing nuclear swelling and decreasing chromatin condensation, thereby rendering DNA more accessible to the replication machinery.
Note: Reproducció del document publicat a: http://dx.doi.org/10.1529/biophysj.107.116863
It is part of: Biophysical Journal, 2008, vol. 94, núm. 12, p. 4984-4995
Related resource: http://dx.doi.org/10.1529/biophysj.107.116863
URI: http://hdl.handle.net/2445/25802
ISSN: 0006-3495
Appears in Collections:Articles publicats en revistes (Ciències Fisiològiques)

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