Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/25803
Title: Mapping cell-matrix stresses during stretch reveals inelastic reorganization of the cytoskeleton.
Author: Gavara i Casas, Núria
Roca-Cusachs Soulere, Pere
Sunyer, Raimon
Farré Ventura, Ramon
Navajas Navarro, Daniel
Keywords: Citosquelet
Cèl·lules epitelials
Transducció de senyal cel·lular
Cytoskeleton
Epithelial cells
Cellular signal transduction
Issue Date: 2008
Publisher: Biophysical Society
Abstract: The mechanical properties of the living cell are intimately related to cell signaling biology through cytoskeletal tension. The tension borne by the cytoskeleton (CSK) is in part generated internally by the actomyosin machinery and externally by stretch. Here we studied how cytoskeletal tension is modified during stretch and the tensional changes undergone by the sites of cell-matrix interaction. To this end we developed a novel technique to map cell-matrix stresses during application of stretch. We found that cell-matrix stresses increased with imposition of stretch but dropped below baseline levels on stretch release. Inhibition of the actomyosin machinery resulted in a larger relative increase in CSK tension with stretch and in a smaller drop in tension after stretch release. Cell-matrix stress maps showed that the loci of cell adhesion initially bearing greater stress also exhibited larger drops in traction forces after stretch removal. Our results suggest that stretch partially disrupts the actin-myosin apparatus and the cytoskeletal structures that support the largest CSK tension. These findings indicate that cells use the mechanical energy injected by stretch to rapidly reorganize their structure and redistribute tension.
Note: Reproducció del document publicat a: http://dx.doi.org/10.1529/biophysj.107.124180
It is part of: Biophysical Journal, 2008, vol. 95, núm. 1, p. 464-471
Related resource: http://dx.doi.org/10.1529/biophysj.107.124180
URI: http://hdl.handle.net/2445/25803
ISSN: 0006-3495
Appears in Collections:Articles publicats en revistes (Ciències Fisiològiques)

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