Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/33764
Title: Characterization of Arabidopsis FPS isozymes and FPS gene expression analysis provide insight into the biosynthesis of isoprenoid precursors in seeds
Author: Keim, Ana Verónica
Manzano Alías, David
Fernández, Francisco J.
Closa Calvo, Marta
Andrade Poveda, Paola Andrea
Caudepón, Daniel
Bortolotti, Cristina
Vega, M. Cristina
Arró i Plans, Montserrat
Ferrer i Prats, Albert
Keywords: Llavors
Genètica vegetal
Genètica molecular vegetal
Isoprenoides
Àrabis
Seeds
Plant genetics
Plant molecular genetics
Isopentenoids
Arabis
Issue Date: 8-Nov-2012
Publisher: Public Library of Science (PLoS)
Abstract: Arabidopsis thaliana contains two genes encoding farnesyl diphosphate (FPP) synthase (FPS), the prenyl diphoshate synthase that catalyzes the synthesis of FPP from isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). In this study, we provide evidence that the two Arabidopsis short FPS isozymes FPS1S and FPS2 localize to the cytosol. Both enzymes were expressed in E. coli, purified and biochemically characterized. Despite FPS1S and FPS2 share more than 90% amino acid sequence identity, FPS2 was found to be more efficient as a catalyst, more sensitive to the inhibitory effect of NaCl, and more resistant to thermal inactivation than FPS1S. Homology modelling for FPS1S and FPS2 and analysis of the amino acid differences between the two enzymes revealed an increase in surface polarity and a greater capacity to form surface salt bridges of FPS2 compared to FPS1S. These factors most likely account for the enhanced thermostability of FPS2. Expression analysis of FPS::GUS genes in seeds showed that FPS1 and FPS2 display complementary patterns of expression particularly at late stages of seed development, which suggests that Arabidopsis seeds have two spatially segregated sources of FPP. Functional complementation studies of the Arabidopsis fps2 knockout mutant seed phenotypes demonstrated that under normal conditions FPS1S and FPS2 are functionally interchangeable. A putative role for FPS2 in maintaining seed germination capacity under adverse environmental conditions is discussed.
Note: Reproducció del document publicat a: http://dx.doi.org/10.1371/journal.pone.0049109
It is part of: PLoS One, 2012, vol. 7, num. 11, p. e49109
Related resource: http://dx.doi.org/10.1371/journal.pone.0049109
URI: http://hdl.handle.net/2445/33764
ISSN: 1932-6203
Appears in Collections:Articles publicats en revistes (Bioquímica i Biomedicina Molecular)

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