Please use this identifier to cite or link to this item:
http://hdl.handle.net/2445/33764
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Keim, Ana Verónica Beatriz | - |
dc.contributor.author | Manzano Alías, David | - |
dc.contributor.author | Fernández, Francisco J. | - |
dc.contributor.author | Closa Calvo, Marta | - |
dc.contributor.author | Andrade Poveda, Paola Andrea | - |
dc.contributor.author | Caudepón, Daniel | - |
dc.contributor.author | Bortolotti, Cristina | - |
dc.contributor.author | Vega Fernández, Maria Cristina | - |
dc.contributor.author | Arró i Plans, Montserrat | - |
dc.contributor.author | Ferrer i Prats, Albert | - |
dc.date.accessioned | 2013-02-08T09:12:13Z | - |
dc.date.available | 2013-02-08T09:12:13Z | - |
dc.date.issued | 2012-11-08 | - |
dc.identifier.issn | 1932-6203 | - |
dc.identifier.uri | http://hdl.handle.net/2445/33764 | - |
dc.description.abstract | Arabidopsis thaliana contains two genes encoding farnesyl diphosphate (FPP) synthase (FPS), the prenyl diphoshate synthase that catalyzes the synthesis of FPP from isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). In this study, we provide evidence that the two Arabidopsis short FPS isozymes FPS1S and FPS2 localize to the cytosol. Both enzymes were expressed in E. coli, purified and biochemically characterized. Despite FPS1S and FPS2 share more than 90% amino acid sequence identity, FPS2 was found to be more efficient as a catalyst, more sensitive to the inhibitory effect of NaCl, and more resistant to thermal inactivation than FPS1S. Homology modelling for FPS1S and FPS2 and analysis of the amino acid differences between the two enzymes revealed an increase in surface polarity and a greater capacity to form surface salt bridges of FPS2 compared to FPS1S. These factors most likely account for the enhanced thermostability of FPS2. Expression analysis of FPS::GUS genes in seeds showed that FPS1 and FPS2 display complementary patterns of expression particularly at late stages of seed development, which suggests that Arabidopsis seeds have two spatially segregated sources of FPP. Functional complementation studies of the Arabidopsis fps2 knockout mutant seed phenotypes demonstrated that under normal conditions FPS1S and FPS2 are functionally interchangeable. A putative role for FPS2 in maintaining seed germination capacity under adverse environmental conditions is discussed. | - |
dc.format.extent | 19 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Public Library of Science (PLoS) | - |
dc.relation.isformatof | Reproducció del document publicat a: http://dx.doi.org/10.1371/journal.pone.0049109 | - |
dc.relation.ispartof | PLoS One, 2012, vol. 7, num. 11, p. e49109 | - |
dc.relation.uri | http://dx.doi.org/10.1371/journal.pone.0049109 | - |
dc.rights | cc-by (c) Keim, Ana Verónica et al., 2012 | - |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es | - |
dc.source | Articles publicats en revistes (Bioquímica i Biomedicina Molecular) | - |
dc.subject.classification | Llavors | - |
dc.subject.classification | Genètica vegetal | - |
dc.subject.classification | Genètica molecular vegetal | - |
dc.subject.classification | Isoprenoides | - |
dc.subject.classification | Àrabis | - |
dc.subject.other | Seeds | - |
dc.subject.other | Plant genetics | - |
dc.subject.other | Plant molecular genetics | - |
dc.subject.other | Isopentenoids | - |
dc.subject.other | Arabis | - |
dc.title | Characterization of Arabidopsis FPS isozymes and FPS gene expression analysis provide insight into the biosynthesis of isoprenoid precursors in seeds | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.idgrec | 617976 | - |
dc.date.updated | 2013-02-08T09:12:13Z | - |
dc.relation.projectID | info:eu-repo/grantAgreement/EC/FP7/279039/EU//COMPLEXINC | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 23145086 | - |
Appears in Collections: | Articles publicats en revistes (Bioquímica i Biomedicina Molecular) |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
617976.pdf | 1.5 MB | Adobe PDF | View/Open |
This item is licensed under a Creative Commons License