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dc.contributor.authorMetón Teijeiro, Isidoro-
dc.contributor.authorMediavilla Redondo, Dominica-
dc.contributor.authorCaseras Surribas, Anna-
dc.contributor.authorCantó, Elisabet-
dc.contributor.authorFernández González, Felipe Javier-
dc.contributor.authorVázquez Baanante, Ma. Isabel-
dc.description.abstractThe effects of diet composition and ration size on the activities of key enzymes involved in intermediary metabolism were studied in the liver of gilthead sea bream (Sparus aurata). Highcarbohydrate, low-protein diets stimulated 6-phosphofructo 1-kinase (EC, pyruvate kinase (EC, glucose-6-phosphate dehydrogenase (EC and 6-phosphogluconate dehydrogenase (EC enzyme activities, while they decreased alanine aminotransferase (EC activity. A high degree of correlation was found between food ration size and the activity of the enzymes 6-phosphofructo 1-kinase, pyruvate kinase, glucose-6-phosphate dehydrogenase (positive correlations) and fructose-1,6-bisphosphatase (EC (negative correlation). These correlations matched well with the high correlation also found between ration size and growth rate in starved fish refed for 22 d. Limited feeding (5 g/kg body weight) for 22 d decreased the activities of the key enzymes for glycolysis and lipogenesis, and alanine aminotransferase activity. The findings presented here indicate a high level of metabolic adaptation to both diet type and ration size. In particular, adaptation of enzyme activities to the consumption of a diet with a high carbohydrate level suggests that a carnivorous fish like Sparus aurata can tolerate partial replacement of protein by carbohydrate in the commercial diets supplied in culture. The relationship between enzyme activities, ration size and fish growth indicates that the enzymes quickly respond to dietary manipulations of cultured fish.-
dc.format.extent10 p.-
dc.publisherCambridge University Press-
dc.relation.ispartofBritish Journal of Nutrition, 1999, vol. 82, num. 3, p. 223-232-
dc.rights(c) Cambridge University Press, 1999-
dc.subject.otherSparus aurata-
dc.subject.otherAmino acids-
dc.titleEffect of diet composition and ration size on key enzyme activities of glycolysis-gluconeogenesis, pentose phosphate pathway and amino acid metabolism in liver of Sparus aurataeng
Appears in Collections:Articles publicats en revistes (Bioquímica i Biomedicina Molecular)
Articles publicats en revistes (Biologia Evolutiva, Ecologia i Ciències Ambientals)

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