Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/33809
Title: Planarians as a model to assess in vivo the role of matrix metalloproteinase genes during homeostasis and regeneration
Author: Isolani, M. E.
Abril Ferrando, Josep Francesc, 1970-
Saló i Boix, Emili
Deri, P.
Bianucci, A. M.
Batistoni, R.
Keywords: Regeneració (Biologia)
Cèl·lules mare
Planària (Gènere)
Genètica molecular
Regeneration (Biology)
Stem cells
Planaria (Genus)
Molecular genetics
Issue Date: 6-Feb-2013
Publisher: Public Library of Science (PLoS)
Abstract: Matrix metalloproteinases (MMPs) are major executors of extracellular matrix remodeling and, consequently, play key roles in the response of cells to their microenvironment. The experimentally accessible stem cell population and the robust regenerative capabilities of planarians offer an ideal model to study how modulation of the proteolytic system in the extracellular environment affects cell behavior in vivo. Genome-wide identification of Schmidtea mediterranea MMPs reveals that planarians possess four mmp-like genes. Two of them (mmp1 and mmp2) are strongly expressed in a subset of secretory cells and encode putative matrilysins. The other genes (mt-mmpA and mt-mmpB) are widely expressed in postmitotic cells and appear structurally related to membrane-type MMPs. These genes are conserved in the planarian Dugesia japonica. Here we explore the role of the planarian mmp genes by RNA interference (RNAi) during tissue homeostasis and regeneration. Our analyses identify essential functions for two of them. Following inhibition of mmp1 planarians display dramatic disruption of tissues architecture and significant decrease in cell death. These results suggest that mmp1 controls tissue turnover, modulating survival of postmitotic cells. Unexpectedly, the ability to regenerate is unaffected by mmp1(RNAi). Silencing of mt-mmpA alters tissue integrity and delays blastema growth, without affecting proliferation of stem cells. Our data support the possibility that the activity of this protease modulates cell migration and regulates anoikis, with a consequent pivotal role in tissue homeostasis and regeneration. Our data provide evidence of the involvement of specific MMPs in tissue homeostasis and regeneration and demonstrate that the behavior of planarian stem cells is critically dependent on the microenvironment surrounding these cells. Studying MMPs function in the planarian model provides evidence on how individual proteases work in vivo in adult tissues. These results have high potential to generate significant information for development of regenerative and anti cancer therapies.
Note: Reproducció del document publicat a: http://dx.doi.org/10.1371/journal.pone.0055649
It is part of: PLoS One, 2013, vol. 8, num. 2
Related resource: http://dx.doi.org/10.1371/journal.pone.0055649
URI: http://hdl.handle.net/2445/33809
ISSN: 1932-6203
Appears in Collections:Articles publicats en revistes (Genètica, Microbiologia i Estadística)

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