Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/34071
Title: Characterization of an antennal carboxylesterase from the pest moth Spodoptera littoralis degrading a host plant odorant.
Author: Durand, Nicolas
Carot-Sans, Gerard
Chertemps, Thomas
Bozzolan, Françoise
Party, Virginie
Renou, Michel
Debernard, Stéphane
Rosell Pellisé, Glòria
Maïbèche-Coisne, Martine
Keywords: Lepidòpters
Enzims
Metabolisme de les plantes
Relacions hoste-paràsit
Metabolisme de proteïnes
Esterases
Lepidoptera
Enzymes
Plant metabolism
Host-parasite relationships
Protein metabolism
Issue Date: 29-Nov-2010
Publisher: Public Library of Science (PLoS)
Abstract: Background: Carboxyl/cholinesterases (CCEs) are highly diversified in insects. These enzymes have a broad range of proposed functions, in neuro/developmental processes, dietary detoxification, insecticide resistance or hormone/pheromone degradation. As few functional data are available on purified or recombinant CCEs, the physiological role of most of these enzymes is unknown. Concerning their role in olfaction, only two CCEs able to metabolize sex pheromones have been functionally characterized in insects. These enzymes are only expressed in the male antennae, and secreted into the lumen of the pheromone-sensitive sensilla. CCEs able to hydrolyze other odorants than sex pheromones, such as plant volatiles, have not been identified. Methodology: In Spodoptera littoralis, a major crop pest, a diversity of antennal CCEs has been previously identified. We have employed here a combination of molecular biology, biochemistry and electrophysiology approaches to functionally characterize an intracellular CCE, SlCXE10, whose predominant expression in the olfactory sensilla suggested a role in olfaction. A recombinant protein was produced using the baculovirus system and we tested its catabolic properties towards a plant volatile and the sex pheromone components. Conclusion: We showed that SlCXE10 could efficiently hydrolyze a green leaf volatile and to a lesser extent the sex pheromone components. The transcript level in male antennae was also strongly induced by exposure to this plant odorant. In antennae, SlCXE10 expression was associated with sensilla responding to the sex pheromones and to plant odours. These results suggest that a CCE-based intracellular metabolism of odorants could occur in insect antennae, in addition to the extracellular metabolism occurring within the sensillar lumen. This is the first functional characterization of an Odorant- Degrading Enzyme active towards a host plant volatile.
Note: Reproducció del document publicat a: http://dx.doi.org/10.1371/journal.pone.0015026
It is part of: PLoS One, 2010, vol. 5, num. 11, p. e15026
Related resource: http://dx.doi.org/10.1371/journal.pone.0015026
URI: http://hdl.handle.net/2445/34071
ISSN: 1932-6203
Appears in Collections:Articles publicats en revistes (Farmacologia, Toxicologia i Química Terapèutica)

Files in This Item:
File Description SizeFormat 
583099.pdf1.44 MBAdobe PDFView/Open


This item is licensed under a Creative Commons License Creative Commons