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DC Field | Value | Language |
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dc.contributor.author | Chertemps, Thomas | - |
dc.contributor.author | François, Adrien | - |
dc.contributor.author | Durand, Nicolas | - |
dc.contributor.author | Rosell Pellisé, Glòria | - |
dc.contributor.author | Dekker, Teun | - |
dc.contributor.author | Lucas, Philippe | - |
dc.contributor.author | Maïbèche-Coisne, Martine | - |
dc.date.accessioned | 2013-04-10T17:05:18Z | - |
dc.date.available | 2013-04-10T17:05:18Z | - |
dc.date.issued | 2012-06-21 | - |
dc.identifier.issn | 1741-7007 | - |
dc.identifier.uri | http://hdl.handle.net/2445/34504 | - |
dc.description.abstract | Background: Insects respond to the spatial and temporal dynamics of a pheromone plume, which implies not only a strong response to"odor on", but also to"odor off". This requires mechanisms geared toward a fast signal termination. Several mechanisms may contribute to signal termination, among which odorant-degrading enzymes. These enzymes putatively play a role in signal dynamics by a rapid inactivation of odorants in the vicinity of the sensory receptors, although direct in vivo experimental evidences are lacking. Here we verified the role of an extracellular carboxylesterase, esterase-6 (Est-6), in the sensory physiological and behavioral dynamics of Drosophila melanogaster response to its pheromone, cis-vaccenyl acetate (cVA). Est-6 was previously linked to post-mating effects in the reproductive system of females. As Est-6 is also known to hydrolyze cVA in vitro and is expressed in the main olfactory organ, the antenna, we tested here its role in olfaction as a putative odorant-degrading enzyme. Results: We first confirm that Est-6 is highly expressed in olfactory sensilla, including cVA-sensitive sensilla, and we show that expression is likely associated with non-neuronal cells. Our electrophysiological approaches show that the dynamics of olfactory receptor neuron (ORN) responses is strongly influenced by Est-6, as in Est-6° null mutants (lacking the Est-6 gene) cVA-sensitive ORN showed increased firing rate and prolonged activity in response to cVA. Est-6° mutant males had a lower threshold of behavioral response to cVA, as revealed by the analysis of two cVAinduced behaviors. In particular, mutant males exhibited a strong decrease of male-male courtship, in association with a delay in courtship initiation. Conclusions: Our study presents evidence that Est-6 plays a role in the physiological and behavioral dynamics of sex pheromone response in Drosophila males and supports a role of Est-6 as an odorant-degrading enzyme (ODE) in male antennae. Our results also expand the role of Est-6 in Drosophila biology, from reproduction to olfaction, and highlight the role of ODEs in insect olfaction. Keywords: carboxylesterase, esterase 6, olfaction, pheromone, signal termination | - |
dc.format.extent | 12 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | BioMed Central | - |
dc.relation.isformatof | Reproducció del document publicat a: http://dx.doi.org/10.1186/1741-7007-10-56 | - |
dc.relation.ispartof | Bmc Biology, 2012, vol. 10, num. 56 | - |
dc.relation.uri | http://dx.doi.org/10.1186/1741-7007-10-56 | - |
dc.rights | cc-by (c) Chertemps, Thomas et al., 2012 | - |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es | - |
dc.source | Articles publicats en revistes (Farmacologia, Toxicologia i Química Terapèutica) | - |
dc.subject.classification | Drosòfila | - |
dc.subject.classification | Enzims | - |
dc.subject.classification | Feromones | - |
dc.subject.classification | Etologia | - |
dc.subject.classification | Olors | - |
dc.subject.classification | Esterases | - |
dc.subject.other | Drosophila | - |
dc.subject.other | Enzymes | - |
dc.subject.other | Pheromones | - |
dc.subject.other | Animal behavior | - |
dc.subject.other | Odors | - |
dc.subject.other | Esterases | - |
dc.title | A carboxylesterase, Esterase-6, modulates sensory physiological and behavioural response dynamics to pheromone in Drosophila | eng |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.idgrec | 590008 | - |
dc.date.updated | 2013-04-10T17:05:19Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 22715942 | - |
Appears in Collections: | Articles publicats en revistes (Farmacologia, Toxicologia i Química Terapèutica) |
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