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Title: Microscopic, chemical, and molecular-biological investigation of the decayed medieval stained window glasses of two Catalonian churches
Author: Piñar G.
Garcia-Valles, Maite
Gimeno Torrente, Domingo
Fernández Turiel, José Luis
Ettenauer, J.
Sterflinger, K.
Keywords: Vitralls
Glass painting and staining
Issue Date: 2012
Publisher: Elsevier B.V.
Abstract: We investigated the decayed historical church window glasses of two Catalonian churches, both under Mediterranean climate. Glass surfaces were studied by scanning electron microscopy (SEM), energy dispersive spectrometry (EDS), and X-ray diffraction (XRD). Their chemical composition was determined by avelength-dispersive spectrometry (WDS) microprobe analysis. The biodiversity was investigated by molecular methods: DNA extraction from glass, amplification by PCR targeting the16S rRNA and ITS regions, and fingerprint analyses by denaturing gradient gel electrophoresis (DGGE). Clone libraries containing either PCR fragments of the bacterial 16S rDNA or the fungal ITS regions were screened by DGGE. Clone inserts were sequenced and compared with the EMBL database.
Note: Versió postprint del document publicat a: 10.1016/j.ibiod.2012.02.008
It is part of: International Biodeterioration and Biodegradation, 2012, p. 1-13
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ISSN: 0964-8305
Appears in Collections:Articles publicats en revistes (Mineralogia, Petrologia i Geologia Aplicada)

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