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Title: | Mouse hepatic oval cells require Met-dependent PI3K to impair TGF-β-Induced oxidative stress and apoptosis. |
Author: | Martínez Palacián, Adoración Castillo, Gaelle del Suárez Causado, Amileth García Álvaro, María Morena Frutos, Diego de la Fernández, Margarita Roncero, Cesáreo Fabregat Romero, Isabel Herrera, Blanca Sánchez, Aránzazu |
Keywords: | Apoptosi Estrès oxidatiu Cèl·lules hepàtiques Apoptosis Oxidative stress Liver cells |
Issue Date: | 2-Jan-2013 |
Publisher: | Public Library of Science (PLoS) |
Abstract: | We have previously shown that oval cells harboring a genetically inactivated Met tyrosine kinase (Met−/− oval cells) are more sensitive to TGF-β-induced apoptosis than cells expressing a functional Met (Metflx/flx), demonstrating that the HGF/Met axis plays a pivotal role in oval cell survival. Here, we have examined the mechanism behind this effect and have found that TGF-β induced a mitochondria-dependent apoptotic cell death in Metflx/flx and Met−/− oval cells, associated with a marked increase in levels of the BH3-only proteins Bim and Bmf. Bmf plays a key role during TGF-β-mediated apoptosis since knocking down of BMF significantly diminished the apoptotic response in Met-/- oval cells. TGF-β also induced oxidative stress accompanied by NADPH oxidase 4 (Nox4) mRNA up-regulation and decreased protein levels of antioxidant enzymes. Antioxidants inhibit both TGF-β-induced caspase 3 activity and Bmf up-regulation, revealing an oxidative stress-dependent Bmf regulation by TGF-β. Notably, oxidative stress-related events were strongly amplified in Met−/− oval cells, emphasizing the critical role of Met in promoting survival. Pharmacological inhibition of PI3K did impair HGF-driven protection from TGF-β-induced apoptosis and increased sensitivity of Metflx/flx oval cells to TGF-ß by enhancing oxidative stress, reaching apoptotic indices similar to those obtained in Met−/− oval cells. Interestingly, both PI3K inhibition and/or knockdown itself resulted in caspase-3 activation and loss of viability in Metflx/flx oval cells, whereas no effect was observed in Met−/− oval cells. Altogether, results presented here provide solid evidences that both paracrine and autocrine HGF/Met signaling requires PI3K to promote mouse hepatic oval cell survival against TGF-β-induced oxidative stress and apoptosis. |
Note: | Reproducció del document publicat a: http://dx.doi.org/10.1371/journal.pone.0053108 |
It is part of: | PLoS One, 2013, vol. 8, num. 1, p. 1-14 |
URI: | http://hdl.handle.net/2445/36385 |
Related resource: | http://dx.doi.org/10.1371/journal.pone.0053108 |
ISSN: | 1932-6203 |
Appears in Collections: | Articles publicats en revistes (Ciències Fisiològiques) Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) |
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