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Title: Reversible mitochondrial respiratory chain impairment during symptomatic hyperlactatemia associated with antiretroviral therapy
Author: Miró, Òscar
López, Sònia
Martínez, Esteban
Rodríguez Santiago, Benjamín
Blanco, José L.
Milinkovic, Ana
Miró Meda, José M.
Nunes Martínez, Virginia
Casademont i Pou, Jordi
Gatell, José M.
Cardellach, Francesc
Keywords: Antiretrovirals
ADN mitocondrial
Antiretroviral agents
Mitochondrial DNA
Issue Date: Nov-2003
Publisher: Mary Ann Liebert, Inc.
Abstract: Direct evidence confirming the hypothesis that a dysfunction of the mitochondrial respiratory chain (MRC) underlies the pathogenesis of hyperlactatemia associated with highly active antiretroviral therapy (HAART) is scarce. We studied mitochondrial DNA (mtDNA) content and MRC function in the skeletal muscle of an HIV-infected patient during an episode of symptomatic hyperlactatemia. Skeletal muscle biopsy was performed during the episode when the patient was symptomatic and 3 months later when the patient was clinically recovered. Assessment of mitochondria was performed using histological, polarographic, spectrophotometrical, and Southern blot and real time PCR DNA quantification methods. The histological study disclosed extensive mitochondrial impairment in the form of ragged-red fibers or equivalents on oxidative reactions. These findings were associated with an increase in mitochondrial content and a decrease in both mitochondrial respiratory capacity and MRC enzyme activities. Mitochondrial DNA content declined to 53% of control values. Mitochondrial abnormalities had almost disappeared later when the patient became asymptomatic. Our findings support the hypothesis that MRC dysfunction stands at the basis of HAART-related hyperlactatemia.
Note: Reproducció del document publicat a:
It is part of: Aids Research and Human Retroviruses, 2003, vol. 19, num. 11, p. 1027-1032
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ISSN: 0889-2229
Appears in Collections:Articles publicats en revistes (Ciències Fisiològiques)
Articles publicats en revistes (Medicina)

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