Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/42037
Title: Transketolase-like 1 expression is modulated during Colorectal cancer progression and metastasis formation
Author: Diaz Moralli, Santiago
Tarrado Castellarnau, Míriam
Alenda, Cristina
Castells Garangou, Antoni
Cascante i Serratosa, Marta
Keywords: Càncer
Càncer colorectal
Metàstasi
Cancer
Colorectal cancer
Metastasis
Issue Date: 2011
Publisher: Public Library of Science (PLoS)
Abstract: Background Transketolase-like 1 (TKTL1) induces glucose degradation through anaerobic pathways, even in presence of oxygen, favoring the malignant aerobic glycolytic phenotype characteristic of tumor cells. As TKTL1 appears to be a valid biomarker for cancer prognosis, the aim of the current study was to correlate its expression with tumor stage, probability of tumor recurrence and survival, in a series of colorectal cancer patients. Methodolody/Principal Findings Tumor tissues from 63 patients diagnosed with colorectal cancer at different stages of progression were analyzed for TKTL1 by immunohistochemistry. Staining was quantified by computational image analysis, and correlations between enzyme expression, local growth, lymph-node involvement and metastasis were assessed. The highest values for TKTL1 expression were detected in the group of stage III tumors, which showed significant differences from the other groups (Kruskal-Wallis test, P = 0.000008). Deeper analyses of T, N and M classifications revealed a weak correlation between local tumor growth and enzyme expression (Mann-Whitney test, P = 0.029), a significant association of the enzyme expression with lymph-node involvement (Mann-Whitney test, P = 0.0014) and a significant decrease in TKTL1 expression associated with metastasis (Mann-Whitney test, P = 0.0004). Conclusions/Significance To our knowledge, few studies have explored the association between variations in TKTL1 expression in the primary tumor and metastasis formation. Here we report downregulation of enzyme expression when metastasis appears, and a correlation between enzyme expression and regional lymph-node involvement in colon cancer. This finding may improve our understanding of metastasis and lead to new and more efficient therapies against cancer.
Note: Reproducció del document publicat a: http://dx.doi.org/10.1371/journal.pone.0025323
It is part of: PLoS One, 2011, vol. 6, num. 9, p. e25323-e25323
Related resource: http://dx.doi.org/10.1371/journal.pone.0025323
URI: http://hdl.handle.net/2445/42037
ISSN: 1932-6203
Appears in Collections:Articles publicats en revistes (Bioquímica i Biomedicina Molecular)

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