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Title: Evidence for transcript networks composed of chimeric RNAs in human cells
Author: Djebali, Sarah
Lagarde, Julien
Kapranov, Philipp
Lacroix, Vincent
Borel, Christelle
Mudge, Jonathan M.
Howald, Cédric
Foissac, Sylvain
Ucla, Catherine
Chrast, Jacqueline
Ribeca, Paolo
Martín, David
Murray, Ryan R.
Yang, Xinping
Ghamsari, Lila
Lin, Chenwei
Bell, Ian
Dumais, Erica
Gelpí Buchaca, Josep Lluís
Orozco López, Modesto
Keywords: RNA
Transcripció genètica
Genètica molecular
Genetic transcription
Molecular genetics
Issue Date: Jan-2012
Publisher: Public Library of Science (PLoS)
Abstract: The classic organization of a gene structure has followed the Jacob and Monod bacterial gene model proposed more than 50 years ago. Since then, empirical determinations of the complexity of the transcriptomes found in yeast to human has blurred the definition and physical boundaries of genes. Using multiple analysis approaches we have characterized individual gene boundaries mapping on human chromosomes 21 and 22. Analyses of the locations of the 5′ and 3′ transcriptional termini of 492 protein coding genes revealed that for 85% of these genes the boundaries extend beyond the current annotated termini, most often connecting with exons of transcripts from other well annotated genes. The biological and evolutionary importance of these chimeric transcripts is underscored by (1) the non-random interconnections of genes involved, (2) the greater phylogenetic depth of the genes involved in many chimeric interactions, (3) the coordination of the expression of connected genes and (4) the close in vivo and three dimensional proximity of the genomic regions being transcribed and contributing to parts of the chimeric RNAs. The non-random nature of the connection of the genes involved suggest that chimeric transcripts should not be studied in isolation, but together, as an RNA network.
Note: Reproducció del document publicat a:
It is part of: PLoS One, 2012, vol. 7, num. 1, p. e28213
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ISSN: 1932-6203
Appears in Collections:Articles publicats en revistes (Bioquímica i Biomedicina Molecular)
Articles publicats en revistes (Institut de Recerca Biomèdica (IRB Barcelona))

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